病毒性心肌炎与扩张型心肌病心肌线粒体DNA缺失的定量分析  

Quantitative analysis of mitochondrial DNA deletions in myocardial biopsy samples from patients with viral myocarditis and dilated cardiomyopathy

作　　者：魏瑾[1] 刘治全[2] 王文茂[2] 袁祖贻[2] 牛小麟[1] 刘昀[1] 

机构地区：[1]西安医科大学第二附属医院心内科,710004 [2]西安医科大学第一附属医院心内科

出　　处：《中华心血管病杂志》2001年第5期283-285,共3页Chinese Journal of Cardiology

基　　金：国家"九.五"科技攻关项目

摘　　要：目的　对比研究病毒性心肌炎 (VMC)与扩张型心肌病 (DCM)患者活检心肌组织线粒体DNA(mtDNA)缺失突变情况及其与外周淋巴细胞mtDNA缺失程度的相关性。方法　用定量PCR法检测 2 0例VMC患者、12例DCM患者心肌细胞及其外周血淋巴细胞mtDNA4977碱基对 (mtDNA4 977)和mtDNA7436碱基对 (mtDNA74 3 6)缺失率。取 12例健康意外死亡者心肌和 2 3例献血员外周血淋巴细胞作正常对照。结果　正常对照者、VMC和DCM患者心肌细胞均存在mtDNA4 977及mtDNA74 3 6缺失 ,合计缺失率分别为 0 .175 %、0 .385 %和 3.0 0 4% ;外周淋巴细胞mtDNA缺失程度与心肌细胞呈一致性改变 ,且有良好的相关性 (r=0 .96 0 ,P <0 .0 0 1)。结论　mtDNA缺失可能是VMC发病及其向DCM演变的一个重要心肌损伤机制 ;外周淋巴细胞在研究心肌细胞mtDNA缺失中的作用值得进一步探讨。Objective To investigate the mitochondrial DNA (mtDNA) deletions in myocardial biopsy samples in patients with viral myocarditis (VMC) and dilated cardiomyopathy (DCM),and the relation between mtDNA deletions and peripheral lymphocytes. Methods mtDNA4977 base pair (mtDNA 4977 )and mtDNA7436 base pair(mtDNA 7436 )deletion rates of myocytes and lymphocytes were measured by the method of quantitative PCR in 20 VMC patients, 12 DCM patients and 35 control cases (12 myocardial samples from the healthy cases died of accident, 23 blood samples of lymphocytes from the blood donators). Results mtDNA 4977 and mtDNA 7436 deletions were observed in both controls (0.175%) and patients with VMC (0.385%) and DCM(3.004%). The severity of mtDNA deletions in VMC and DCM cases were 1.2 and 16.2 times higher than in normal subjects. mtDNA deletion rates in patients with DCM were 6.8 times higher than that in patients with VMC ( P <0.05). The degree of mtDNA deletions in peripheral lymphocyte was similar with and showed well correlation with that in myocardium ( r =0.960, P <0.001). Conclusions mtDNA deletions in myocardium might play an important role in the pathogenesis of VMC as well as its development to DCM. The value of peripheral lymphocyte in the study of myocardial mtDNA deletion needs to be further investigated.

关 键 词：心肌炎 充血性心肌病 线粒体DNA 基因 

分 类 号：R542.21[医药卫生—心血管疾病]