转双抗虫基因741杨特征及其繁殖技术  

THE CHARACTERISTIC OF TWO INSECT-RESISTANT GENES TRANSFORMED HYBRID POPLAR 741 AND ITS PROPAGATION METHODS

作　　者：郑均宝[1] 裴保华[1] 梁海永[1] 王进茂[1] 王永芳[1] 杜克久[1] 杨敏生[1] 高宝嘉[1] 田颖川[2] 贾燕涛[2] 虞红梅[2] 马玉湖 马玉行 

机构地区：[1]河北农业大学林学院,保定071000 [2]中国科学院微生物研究所,北京100018 [3]深州市北四王庄,052800

出　　处：《河北林果研究》2001年第2期97-117,共21页Hebei Journal of Forestry and Orchard Research

基　　金：河北省自然科学基金项目! (编号 :3980 91)

摘　　要：简述了当今植物抗虫基因工程的研究进展 ,介绍了Bt基因和蛋白酶抑制剂基因 ,农杆菌介导的遗传转化以及防治害虫产生抗性的策略等。双抗虫基因是用部分改造BtCry1Ac基因和慈菇蛋白酶抑制剂基因 (API)构建的植物表达载体。被转化体 74 1杨 (Populus×aldatomen tosaCl.74 1)是以银白杨× (山杨 +小叶杨 )为母本 ,毛白杨为父本 ,1994年杂交育成 ,为一优良白杨无性系 ,形态酷似毛白杨。在转双抗虫基因的研究中 ,选出高抗虫、中抗虫无性系。高抗虫无性系杀死昆虫幼虫的总死亡率为 83%～ 90 %。从苗期高生长和形态观察 ,转双抗虫基因 74 1杨生长发育正常。比较详细地介绍了 74 1杨营养繁殖技术及基本原理。介绍了硬枝扦插容器育苗、组培微繁技术。We reviewed briefly the progress of genetic engineering on insect-resistant plants, especially on the Bt gene, the API gene, the agrobacterium-mediated transformation method and the ideas of preventing the resistance formation of insects. The expression vector pBtiA containing the two insect-resistant genes, BtCry1Ac gene and API gene, was introduced into the hybrid poplar 741(Populus×aldatomentosa Cl 741),which was obtained in 1994 by hybridizing P alba L ×(P davidianaDode+P simonii Carr ) with P.tomentosa Carr . The hybrid poplar 741 was a good clone. Its characteristics are very similar to that of P tomentosa Carr . The high insect-resistant clone and the middle insect-resistant clone were selected in insect feeding tests. The larvae mortality rate of the high insect-resistant clone was 83%～90%. The gene-transformed hybrid poplar 741 expressed normally on morphology during its seedling phase. The asexual propagation including cutting, tissue culture, and related principles and skills were attentively introduced in the paper.

关 键 词：双抗虫基因 741杨 遗传转化 繁殖 特征 

分 类 号：S792.11[农业科学—林木遗传育种]