用小卫星探针33.6对中华绒螯蟹遗传多态性的DNA指纹图谱研究  

Genetic polymorphism of Chinese mitten-handed crabs (Eriocheir sinensis) with DNA fingerprinting produced by minisatellite probe 33.6

作　　者：王进科[1] 周刚[2] 曹文明[2] 胡本龙 刑益于 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]江苏省淡水水产研究所,江苏南京210096 [3]江苏省高淳长江水系中华绒螯蟹原种场,江苏高淳211300

出　　处：《大连水产学院学报》2001年第2期92-98,共7页Journal of Dalian Fisheries University

基　　金：江苏省科委"九五"农业科技攻关项目! (BE9642 2 -1 )

摘　　要：运用DIG标记的人源小卫星探针 33 6与用HaeⅢ消化的中华绒螯蟹 (Eriocheirsinensis)基因组DNA杂交 ,进行了中华绒螯蟹种群遗传多态性的非放射性DNA指纹图谱研究。实验表明 :用人源小卫星探针 33 6在中华绒螯蟹DNA指纹图谱中可检测到 1 0个以上位点的遗传变异 ,这些位点均表现丰富的多态性 ;人源小卫星探针 33 6产生的中华绒螯蟹DNA指纹图谱具有个体特异性 ,但未表现出中华绒螯蟹种或种群特异的分子遗传标记 ,长江蟹、辽河蟹与人工繁育长江蟹的群体内相似指数分别为 0 2 92、 0 375和0 30 6;长江蟹与辽河蟹、长江蟹与人工繁育长江蟹、辽河蟹与人工繁育长江蟹群体间相似指数分别为 0 1 78、0 2 66和 0 1 79。The genetic polymorphism of Chinese mitten-handed crabs (Eriocheir sinensis) with non radioactive DNA fingerpringting was studied by hybridizing Hae Ⅲ completely digested genomic DNA of Chinese mitten-handed crabs with DIG labeled probe of human mini satellite 33.6. It was found that DNA fingerprints detected over 10 polymorphic locuses showed individual specificity. However, fingerprints did not show the population specific molecular marker. The genetic similarity index of natural Yangtze River crab population, natural Liaohe River crab population and artificial Yangtze River crab population was 0.292, \{0.375\} and 0.306, respectively. The genetic similarities between population of Yangtze River and Liaohe River, Yangtze River and artificial Yangtze River, Liaohe River and Yangtze River crabs were 0.178 , 0.266 and 0.179, respectively.

关 键 词：中华绒螯蟹 遗传多态性 DNA指纹图谱 小卫星探针 

分 类 号：Q949.223.5[生物学—植物学] S966.16[农业科学—水产养殖]