大熊猫精液的研究  

Studies on spermatozoa in giant pandas

作　　者：黄炎[1] 李德生[1] 张和民[1] 杜军[1] 张贵权[1] 王鹏彦[2] JoGayle Howard Rebecca Spindler 

机构地区：[1]中国保护大熊猫研究中心,四川汶川623006 [2]美国华盛顿国家动物园

出　　处：《中国兽医杂志》2001年第6期9-11,共3页Chinese Journal of Veterinary Medicine

摘　　要：在 1999年 3月至 2 0 0 0年 6月 ,对卧龙自然保护区大熊猫研究中心的 9只 5 .5～ 16 .5岁的雄性大熊猫进行了 17次电刺激采精 ,9只大熊猫的平均体重为 10 8.1± 15 kg,睾丸体积为 2 89.4± 10 8.6 cm3,刺激电压 2～ 8V,射精电压 4～ 8V,平均每次采精量为 3.5± 1.91ml,精子密度为 995 .2± 782 .6× 10 6 个 / ml,每次采精总量为 390 7± 2 981.7× 10 6 个 ,精液平均活力为 6 9.1± 2 8.9% ,活率为 79.6± 2 3.1% ,运动状态为 2 .7± 1.1,畸形率为 39.4± 32 .2 % ,平均顶体正常率为 85 .8± 31.2 % ,p H值为 7.9± 0 .4。其中 3只是能进行自然交配并繁殖了后代的种公兽 ,其体重为 12 2 .7± 7.1kg,睾丸体积为 381.7± 6 4.8cm3,精液活力为 77.3± 5 .8% ,运动状态为 3.0± 0 .5 ,精子畸形率为 18.0± 9.6 % ,顶体正常率为 92 .7± 1.5 % ,p H值为 8.0±0 .1。大熊猫异常精子主要有 :顶体异常 ,卷尾 ,中段弯曲含胞浆小滴 ,中段弯曲无胞浆小滴 ,尾弯曲含胞浆小滴 ,尾弯曲无胞浆小滴 ,近端含胞浆小滴 ,远端含胞浆小滴 8种。在 2月～ 6月都能通过电刺激采集到精液 ,在 4月份每次产精量最高 ,这正好与在这期间发情和交配的大熊猫数量最多相吻合。Nine male pandas between 5.5 to 16.5 years old at the Research and Conservation Center for Giant Panda in Wolong Nature Reserve were used for this study.Spermatozoa were collected by electroejaculation.The average body weight of nine males was 108.1±15KG.The testes volume was 289.4± 108.6 cm 3.Total spermatozoa per ejaculate was 3907±2981.7×10 6. The sperm motility was 69.1±28.9%,speed of progression was 2.7±1.1,the percent abnormal sperm count was 39.4±32.2%,normal acrosome count was 85.8±31.2%,pH was 7.9±0.4.Three of the males already have produced offspring through natural mating. The average weight of these three males was 122.7±7.1KG.Their average testes volume was 381.7±64.7cm 3.Their sperm motility was 77.3±5.8%,status was 3.0±0.5,average percentage of abnormal sperm was 18±9.6%,normal acrosome was 92.7±1.5%,pH was 8.0±0.1.Abnormalities of giant pandas' spermatozoa ware including:abnormal acrosome,tightly coiled tail,bent midpiece w/droplet,bent midpiece w/o droplet,bent tail w/droplet,bent tail w/o droplet,proximal droplet,distal droplet.

关 键 词：大熊猫 电刺激采精 精液 活力 畸形率 

分 类 号：S865.31[农业科学—野生动物驯养]