编码EIAV核输出因子蛋白Rev的cDNA克隆和序列测定  

Cloning and Sequencing of cDNA Encoding Equine Infectious Anemia Virus Rev Protein

作　　者：孔宪刚[1] 张宝山[1] 周家喜[1] 刘永刚[1] 刘胜旺[1] 刘相东[1] 孙成群[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《中国预防兽医学报》2001年第4期241-243,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：EIAV在繁殖过程中涉及到多种转录因子的调节 ,其中Rev蛋白是病毒编码的转录后调节因子 ,决定了病毒复制由早期到晚期的过渡。Rev是非结构蛋白 ,由病毒基因组全长转录产物经多次拼接而成。本研究使用EIAV疫苗毒感染驴巨噬细胞培养物 ,在病毒增殖早期提取总RNA ,反转录后使用EIAV特异引物扩增病毒基因组拼接产物。将扩增产物克隆后经过核苷酸序列分析 ,通过与基因组全序列的比较 。Replication of EIAV is regulated by several transcription factors.Rev is encoded by multispliced genomic mRNA,which switches the virus gene expression from early stage to late stage.In this study,donkey macrophage culture was infected with donkey leukocyte attenuated EIAV;total cellular RNA was extracted from cells at the early stage of the infection.After reverse transcription directed by EIAV specific primer,polymerase chain reaction was performed.Then the amplified segment was cloned and sequenced.By comparison with the viral genome,ORF of rev and splicing sites were determined.

关 键 词：马传贫病毒 Rev蛋白 拼接 CDNA克隆 序列分析 核输出因子 

分 类 号：S852.652[农业科学—基础兽医学]