鸡α干扰素基因的克隆和鉴定  

Cloning and Identification of Chicken Interferon-α Gene

作　　者：曹永长[1] 吕英姿[1] 毕英佐[1] 

机构地区：[1]华南农业大学动物科学系,广州510642

出　　处：《中国预防兽医学报》2001年第4期259-261,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：广东省科技攻关项目 (编号 :99M0 42 0 5G)资助

摘　　要：采用反转录_聚合酶链反应技术 ,从传染性囊病病毒感染的鸡胚成纤维细胞中扩增出石岐杂鸡的α干扰素(sqzChIFN_α)基因。将包括sqzChIFN_α编码区的cDNA片断克隆到pBssK载体中 ,并对该片断进行序列测定。核苷酸序列测定结果表明克隆的ChIFN_α基因cDNA片段长度为 72 4bp ,和其他ChIFN_α基因的同源性在 98 1%以上 ,与其它α禽干扰素基因的同源性在 67 4%以上。其中与鸭α干扰素基因的同源性为 67 4% ,与火鸡α干扰素基因的同源性为 89 8%。推导的石岐杂鸡α干扰素氨基酸序列与其它禽类的α干扰素的同源性在 49.2 %以上。其中与白来航鸡α干扰素的同源性为 96 9%～ 99% ,与鸭和火鸡α干扰素同源性分别为 49 2 %和 80 7%。Using reverse transcription_polymerase chain reaction(RT_PCR)technique,Shiqiza broiler interferon_a gene(sqzChIFN_α) was amplified from chick embryo fibroblasts(CEF s)infected with infectious bursal disease virus.The cDNA fragment containing coding region of ChIFN_α gene was cloned into the pBlueScript vector and sequenced.The sequence analysis showed that the length of the cDNA fragment amplified here was 724bp,and the similarities of nucleotide sequence of sqzChIFN_α was over 98.1% to other ChIFN_α,and over 67.4% to IFN_α gene of other avian species.The similarities of the deduced amino acid sequence of sqzChIFN_α were 99%,49.2%and 80.7% to IFN_α of white leghom,duck and turkey,respectively.

关 键 词：鸡 Α干扰素 基因克隆 序列分析 

分 类 号：S831.2[农业科学—畜牧学] Q511[农业科学—畜牧兽医]