串联启动子调控下的t-PAcDNA乳腺定位表达  

t-PAcDNA expression regulated by linking promoter in mammary gland

作　　者：侯玉泽[1] 李银聚[1] 邓雯[1] 赵君[2] 扈荣良[2] 殷震[2] 

机构地区：[1]洛阳农业高等专科学校,河南洛阳471003 [2]解放军军需大学,吉林长春130062

出　　处：《河南农业大学学报》2001年第2期188-191,共4页Journal of Henan Agricultural University

基　　金："8 63"国家计划资助!项目 (Z2 1-0 4-0 3 )

摘　　要：由牛 β Casein启动子与大鼠WAP启动子串联构建了t PAcDNA乳腺定位表达载体pSVL WAPβ PA ,将 3种表达载体pSVL βb PA ,pSVL WAP PA及pSVL WAPβ PA分别注入怀孕家兔乳腺导管中 ,溶圈试验结果显示 ,3种表达载体均能在家兔乳腺中表达 ,且以分娩后第 4d的乳汁中表达水平最高 ,分别为 390 ,44 0 ,45 0 μg·L-1.本试验为进一步研究t PA基因乳腺定位表达调控和建立tThe specific expression plasmid pSVL WAPβ PA, containing t PA gene, was constructed by linking up bovine β Cansein and WAP promoter. The three plasmids, pSVL β b PA,pSVL WAP PA and pSVL WAPβ PA,were mixed with Liposome and injected directly into pregnant rabbit mammary gland duct. Results of fibrin clot lysis assay showed that the three plasmids could express t PA in rabbit milk. The expression level day 390, 440,450 μg·L -1 respectively reached the highest on the 4th day. This has laid a foundation for further study on the regulation of specific expression in mammary gland and the establishment of t PA mammary gland bioreactor.

关 键 词：Β-酪蛋白基因 乳清秀 酸蛋白基因 调控序列 t-PAcDNA 乳腺 表达 

分 类 号：Q786[生物学—分子生物学]