孕兔子宫内膜淋巴细胞的制备及体外培养  

Preparation and Culture in Vitro of Endometrium Lymphocytes from Pregnant Rabbit

作　　者：沈文正[1] 靳亚平[2] 李引乾[2] 马勇江[2] 

机构地区：[1]杨凌职业技术学院,陕西杨陵712100 [2]西北农林科技大学畜牧兽医学院,陕西杨陵712100

出　　处：《西北农业学报》2001年第2期13-16,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金!资助项目 (编号 :3 9970 5 44)

摘　　要：对孕兔子宫内膜的对比消化及统计分析表明 ,用浓度为 2 .5g/L或 1 .2 5g/L的胰酶 0～ 4℃消化7h3 0 min至 8h55min,可分离到正常的子宫内膜淋巴细胞 ( EML) ;培养条件单独作用或与酶浓度共同作用分别可极显著影响 ( P<0 .0 1 )或显著影响 ( P<0 .0 5)孕兔子宫内膜淋巴细胞的活化作用。试验表明 ,孕兔子宫内膜经 1 .2 5g/L的胰酶 0～ 4℃消化 8～ 9h,所获淋巴细胞在体外培养 4 8h是孕兔子宫内膜淋巴细胞制备及体外培养的可行方案 ;培养出的淋巴细胞适合用噻唑蓝 ( MTT)比色法进行活性检测。Comparative digestion of endometrium lymphocytes (EML) from pregnant rabbits and variance analysis showed that intact EML could be isolated from endometria digested with trypsin(2.5 g/L or 1.25 g/L) for 7 h 30 min to 8 h 55 min(0～4℃) and the activation of the lymphocytes could be affected by culture condition( P <0.01) or by that and trypsin concentration together( P <0.05).It can be concluded that digestion of endometrium with 1.25 g/L trypsin for 8～9 h (0～4℃) was the optimal condition for preparation of EML from pregnant rabbit and the activation of EML obtained could be conveniently tested with MTT assay after 48 hours of culture in vitro.

关 键 词：孕兔 子宫内膜淋巴细胞 消化 体外培养 

分 类 号：S829.1[农业科学—畜牧学]