腺苷预适应对缺血再灌注损伤的保护作用  

The protective effect of adenosine preconditioning on ischaemia-reperfusion injury

作　　者：胡佳乐[1] 李伟[2] 沈甫明[3] 

机构地区：[1]解放军第411医院普外科,上海200081 [2]江苏省人民医院采购中心,南京210000 [3]第二军医大学基础部药理教研室,上海200433

出　　处：《药学实践杂志》2001年第4期206-208,共3页Journal of Pharmaceutical Practice

摘　　要：目的 :观察预适应 (缺血及腺苷 )对大鼠下肢缺血再灌注后心、肺损伤的保护作用。方法 :雄性SD大鼠随机分 4组 ,每组 8只。行腹主动脉下端阻断及再灌注各 12 0min。组Ⅰ为手术对照组。组Ⅱ在缺血再灌注前先行阻断腹主动脉 5min后开放 5min ,共 4次。组Ⅲ麻醉后经颈静脉持续滴注腺苷生理盐水溶液。组Ⅳ为假手术对照组。 4组均于麻醉后经舌静脉注入伊文氏兰 (Evan’sbluedye)溶液 ,动脉插管测定再灌注后的平均动脉压 (MAP)。实验终点取心、肺组织行病理检查 ,心肌ATP酶 ,心、肺组织丙二醛 (malonyldiade hyde ,MDA)含量及Evan氏兰含量测定。 结果 :组Ⅰ、Ⅲ再灌注后MAP均显著低于组Ⅱ、组Ⅳ (P <0 0 5) ,组Ⅰ、Ⅲ间 ,组Ⅱ、Ⅳ间无显著差异。组Ⅰ心肌ATP酶 (包括Na+K+ATPase ,Mg2 +ATPase及Ca2 +AT Pase)活力均低于余 3组 ,余 3组间 2 ,2比较均无显著差异。组Ⅰ心 ,肺组织MDA含量显著高于余 3组 ,余3组间 2 ,2比较均无显著差异。组Ⅰ肺Evan氏兰含量显著高于Ⅱ、Ⅲ、Ⅳ组 ,组Ⅱ、Ⅲ高于组Ⅳ ,组Ⅱ、Ⅲ间无显著差异。结论 :缺血和腺苷预适应能改善大鼠下肢缺血再灌注后心 。OBJECTIVE:To investigate the protective effects of preconditioning on cardiac and pulmonary injury induced by lower extremity ischaemia -reperfusion in rats METHODS: Animals were randomized into 4 groups and underwent laparotomy The abdominal aorta was isolated and clamped below the renal artery level for 120min then declamped for another 120min Group Ⅰ( n =8)was taken as control Group Ⅱ( n =8) was pretreatedwith ischaemia preconditioning that the lower extremities had experienced 4 times of ischaemia and reperfusion 5 min each before the 240 min treatment began Group Ⅲ( n =8) accepted continuous drip infusion of adenosine Group Ⅳ( n =8)was sham operated All groups were given Evan's blue dye intravenously after anesthesia Mean artery pressure ( MAP ), myocardium ATPase activities and malonyldiadehyde ( MDA ), pulmonary MDA and Evan's blue were determined separately RESULTS: MAP was lower in groupⅠand Ⅲ than in Ⅱ and Ⅳ,neither group Ⅰand Ⅲ, norⅡand Ⅳ, had significant differences(Ⅰ:61±14;Ⅱ:86±12; Ⅲ:69±18;Ⅳ:94±23mmHg) Myocardium ATPase activity was lower in group Ⅰthan in group Ⅱ,Ⅲ,Ⅳ(Na +K + ATPase 8 1±0 4 vs 8 9±2 1, 9 4±1 0and 9 0±0 8U/mg; Ca 2+ ATPase 5 0±0 2 vs 5 3±1 0, 5 9±0 7and 5 7±0 8U/mg; Mg 2+ ATPase 4 4±0 3 vs 5 2±0 8, 5 1±0 71and 5 3±0 6 U/mg),the MDA level was higher in groupⅠthan in group Ⅱ,Ⅲ,Ⅳ ( MDA 2 5±0 4 vs 1 8±0 7, 1 6±0 5 and 1 7±0 7nmol/mg) Pulmonary MDA and Evan's blue level were higher in group Ⅰthan in the other three groups( MDA 2 3±0 2vs 1 6±0 4, 1 8±0 5 and 1 4±0 6nmol/mg; Evan's blue 3 1±1 2 vs 1 6±0 5, 1 5±0 3and 0 9±0 2μg/mg) CONCLUSION: Ischaemia and adenosine preconditioning have some protective effects on cardiac and pulmonary injury induced by ischaemia reperfusion of lower extremity in rats

关 键 词：腺苷 缺血 预适应 下肢缺血 再灌注损伤 

分 类 号：R654.4[医药卫生—外科学]