Beaglf犬选择素E基因组DNA的克隆、序列分析及同源性比较  

Cloning, Sequencing of Beagle E selectin Genomic DNA and Comparison of It with Other Species

作　　者：郑立新[1] 石应康[1] 董力[1] 张戈[2] 武辉[2] 李幼平[3] 程述森[1] 

机构地区：[1]华西医科大学附属第一医院胸心血管外科,成都610041 [2]华西医科大学附属第一医院遗传学实验室,成都610041 [3]华西医科大学附属第一医院植免疫实验室,成都610041

出　　处：《华西医科大学学报》2001年第3期325-329,共5页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助 (批准号 3 9870 748)

摘　　要：目的　选择素是细胞粘附分子大家族中的一大类成员 ,在炎性反应及血管生成等诸多方面发挥重要作用。本研究对 BEAGL E犬选择素 E基因组 DNA进行克隆、序列分析及同源性比较。方法　采用分段 PCR的方法 ,经过 PCR扩增、T载体克隆、序列分析、基因拼接及同源性比较等实验步骤。结果　首次获得了具有完整开放阅读框架的 Beagle犬 E- selectin基因组序列 ,通过对其基因结构和同源性比较分析 ,证明该基因在人、猪、犬各种间具有高度的同源性。结论　由于验证了选择素 E基因在种间的高度同源性 。Objective Selectins are a group of members of Cell Adhesion Molecular family; they play very important roles in inflammation and neovascularization. The aim of this study is to clone, sequence Beagle E selectin genomic DNA and make homogeneous analyses to compare it with those of other animals. Methods Twelve fragments of genomic DNA of Beagle E selectin were obtained by PCR, and the 12 fragments were cloned in T vector. At last, the genomic DNA sequences of Beagle E selectin were obtained through gene sequencing,assembling and base calling, and the gene structure was analyzed. Results The sequence of Beagle E selectin genomic DNA that contained the whole open reading box was acgured. The results of homogeneous analyses showed a great extent of E selectin gene homology among human,porcine and dog. Conclusion The demonstration of a large extent of the gene homology has substantiated in this connection the important roles of E selectin in transplantation immunity and neoplasm development.

关 键 词：选择素E 基因组DNA 序列分析 比格尔犬 PCR 克隆 

分 类 号：R346[医药卫生—基础医学]