外周支及中枢支切断对背根节BDNF和NT-3表达的影响  

The Expression of BDNF and NT-3 in Dorsal Root Ganglion Following Peripheral and Central Axotomy-An Immunohistochemical Study

作　　者：倪师今[1] 王廷华[2] 陈彦红[2] 

机构地区：[1]昆明医学院第一附属医院神经外科,昆明650032 [2]昆明医学院神经科学研究所

出　　处：《华西医科大学学报》2001年第3期350-352,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金 (批准号 3 0 0 0 0 2 2 7);云南省自然科学基金 (批准号 99C0 0 0 6 R)资助

摘　　要：目的　探讨中枢支和外周支切断对背根节 (DRG)脑源性神经营养因子 (BDNF)和神经营养因子(NT- 3)表达的影响。方法　将成年雄猫 15只分为正常组 ,外周支切断组及中枢支切断组。后两组分别紧贴背根节切断外周支和中枢支。术后动物存活 4天 ,取各组 L5 DRG用 BDNF和 NT- 3抗血清行 ABC免疫组化染色。计数各组 DRG BDNF和 NT- 3的阳性神经元数。结果　外周支、中枢支切断组 DRG BDNF和 NT- 3的阳性神经元数(2 4.1± 3.5 ,2 9.4± 5 .2、19.4± 2 .4,2 0 .2± 1.9)均较正常组者 (12 .1± 2 .2 ,13.3± 2 .9)明显增多 (P<0 .0 1)。外周支切断者亦较中枢支切断组者增多 (P<0 .0 5 )。结论　外周支及中枢支切断均可致 DRG神经元 BDNF和 NT- 3表达增加。且外周支切断对 DRG神经元 BDNF和 NT-Objective To explore the expression of brain derived neurotrophic factor(BDNF) and neurotrophin 3(NT 3) in dorsal root ganglion (DRG) following peripheral and central axotomy. Methods Fifteen male adult cats were divided into intact group, peripheral axotomy group and central axotomy group. The ten cats of the latter two groups were subjected to unilateral peripheral cut and central processes cut respectively. The animals were allowed to survive 4 days long; L 5 DRG was taken from each animal and made into frozen sections 20 μm thick. The sections were stained using antisera of BDNF and NT 3 respactively by immunohistchemical ABC method. The neuronal numbers of BDNF and NT 3 of the three groups were counted and compared. Results The positive products of BDNF were mainly distributed in small to medium sized neurons (13 57 μm) while those of NT 3 were mainly in large sized neurons (57 100 μm). The neuronal numbers of BDNF and NT 3 in peripheral and central axotomy groups (24.1±3.5, 29.4±5.2; 19.4±2.4, 20.2±1.9) increased significantly than those of intact group (12.1±2.2, 13.3±2.9), P <0.01. And the neuronal numbers of BDNF and NT 3 in peripheral axotomy groups were more than those of central axotomy group( P <0.05). Conclusion\ The results indicate that peripheral axotomy and central axotomy lead to the increase of the expression of BDNF and NT 3 in DRG neurons. And the effect of peripheral axotomy is greater than that of central axotomy.

关 键 词：外周支切断 中枢支切断 脑源性神经营养因子 神经营养因子 背根节 BDNF NF3 

分 类 号：R651.3[医药卫生—外科学]