神经营养素4在面神经的转运研究  

A Study of on the Transport of Neurotrophin 4 in Facial Nerve

作　　者：李云春[1] 杨晓川[1] 李林[1] 王全林[1] 何刚[2] 高炳庆[2] 林代诚[2] 

机构地区：[1]华西医科大学附属第一医院核医学科,成都610041 [2]华西医科大学附属第一医院耳鼻咽喉科,成都610041

出　　处：《华西医科大学学报》2001年第3期353-355,共3页Journal of West China University of Medical Sciences

基　　金：高等学校博士学科点专项科研基金资助 (批准号 96 0 6 3 0 15 )

摘　　要：目的　探讨面神经再生微环境及神经营养素 4(NT- 4)对神经细胞的作用。方法　将新西兰兔一侧面神经干横断后置入硅胶再生室 ,再将 1 3 1 I- NT- 4(10μl/只 ,约 7.4MBq)注入其再生室。另取 1只家兔分别在注药后不同时刻行头部冠状位平面显像 ,其余取面神经干、脑干和对侧面神经干 ,利用放射性核素示踪技术测定 NT- 4在面神经的转运情况。另取 1只置入再生室的兔 ,同时在再生室注入 1m g脑源性神经营养因子 (BDNF)和7.4MBq1 3 1 I- NT- 4后 ,不同时刻行头部冠状位平面显像。结果　注药后 4小时就有 5 .0 8%的 1 3 1 I- NT- 4转运到面神经干中 ,8、12小时达高峰 ,分别为 2 0 .5 8%和 2 2 .74% ;8小时有 34 .75 %转运至脑干 ,12小时转运至脑干的量达45 .5 7% ,且 1 3 1 I- NT- 4在面神经的转运受 BDNF的明显抑制。结论　 NT-Objective This is a study on the transport information of neurotrophin 4 (NT 4) in facial nerve using radioactive tracer technique. Methods After one lateral facial nerve trunk of adult rabbit was transected, a silicone chamber was inserted between the stumps, and 7.4MBq of 131 I NT 4 was administered into the chamber. At the distinct moment of post injection, the head of one rabbit was imaged at coronary position, the bilateral facial nerve trunk and brain stem of the others were collected and counted respectively. After 1mg of brain derived neurotrophic factor (BDNF) and 7.4MBq of 131 I NT 4 were administered into the chamber of one rabbit, the rabbit was imaged at coronary position of head at distinct moment. Results 5.08% of 131 I NT 4 was transported into facial nerve trunk at 4h of post injection. 131 I NT 4 presented high peak in facial nerve trunk during the period of 8,12h, the peak values were 20.58% and 22.74% respectively. 34.75% and 45.57% of 131 I NT 4 were transported into the brain stem of experimental side at 8h and 12h respectively. The tarnsport of 131 I NT 4 was markedly restrained by BDNF in facial nerve. Conclusion NT 4 displays a receptor mediated retrograde transport in facial nerve.

关 键 词：神经营养素4 面神经 受体 转运 面神经再生 

分 类 号：R817.1[医药卫生—影像医学与核医学]