水稻完全显性早熟性的发现和基因定位  

Identification and Gene Mapping of Completely Dominant Earliness in Rice (Oryza sativa L.)

作　　者：邓晓建[1] 周开达[1] 李仁端[1] 淳泽[1] 李平[1] 王文明[2] 翟文学[2] 朱立煌[2] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]中国科学院遗传研究所,北京100101

出　　处：《中国农业科学》2001年第3期233-239,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30070469)

摘　　要：研究发现早釉核不育系６４４２Ｓ－７具有完全显性早熟特性，它与１３个不同类型的中、迟熟品种杂交，Ｆ１抽稳期与６４４２Ｓ－７完全相同或十分相近。对Ｆ２和 Ｂ１Ｆ１遗传分析表明该早熟性主要受２对显性基因控制。利用Ｆ２群体和４种分子标记技术，将６４４２Ｓ－７携带的１个显性早熟基因定位于水稻第３染色体短臂靠近端部一侧，该基因与ＲＡＰＤ标记ＯＰ１１１．５５７、ＳＳＲ标记 ＲＭ２２、ＲＭ２３１、ＲＦＬＰ标记Ｃ５１５和 ＡＦＬＰ标记ＰＴ６７１的遗传距离分别为１．５、３．０、６．７、１０．９和１２．４ｃＭ。该基因系首次发现并定位，暂命名为Ｅｆ－ｃｄ（ｔ）６４４２Ｓ－７作为完全显性早熟性种质资源，对水稻遗传改良具有重要的应用价值。The completely dominant earliness was identified in a genie male- sterile and early maturing indica line 6442S-7. F, progenies from 6442S-7 crossed with thirteen various types of medium-or late-maturing varieties, shared the same heading date as 6442S-7. The segregation of heading date in the F, and B,F, populations showed that the earliness of 6442S-7 is mainly controlled by two dominant major genes. The local linkage map of one dominant earliness gene harbored in 6442S-7 was constructed with F, population and four kinds of molecular marker techniques. The results showed that the gene was located between a RFLP marker C515 and a RAPD marker OPI11. 557 on the short arm of chromosome 3, 10. 9cM and 1. 5 cM from C515 and OPI11. 557, respectively. The genetic distances from the target gene to two SSR markers, RM22 and RM231, and one AFLP marker , PT671, were 3.0, 6.7 and 12.4 cM, respectively. This gene, being identified and mapped first, is designated tentatively as Ef-cd (t). As a new genetic resource of completely dominant earliness, 6442S-7 has splendid future in rice improvement.

关 键 词：水稻 生育期 显性早熟基因 基因定位 

分 类 号：S511[农业科学—作物学]