铁胁迫小麦根DDRT-PCR分析及ABC基因的表达差异  

DDRT-PCR Analysis Under Iron-deficient Condition in Wheat Roots and Difference of ABC Gene Expression

作　　者：印莉萍[1] 刘维仲[1] 刘祥林[1] 黄勤妮[1] 柴东方[1] 邱泽生[1] 王凌诗[2] 

机构地区：[1]首都师范大学生物系,北京100037 [2]北京教育学院,北京100044

出　　处：《中国农业科学》2001年第3期301-303,共3页Scientia Agricultura Sinica

基　　金：北京市自然科学基金资助项目(5982008);国家重点基础研究发展规划项目(973 G1999011700)

摘　　要：采用ＤＤＲＴ－ＰＣＲ技术比较了正常供铁和缺铁胁迫下铁高效型小麦（京－４１１）和铁低效型小麦（三属麦－３）的基因表达差异模式。利用从ＤＤＲＴ－ＰＣＲ中挑选出的ＡＢＣ的ｃＤＮＡ片段为探针进行Ｎｏｒｔｈｅｒｎ杂交。Expression difference of gene in iron-deficiency and iron-sufficiency were compared between iron-efficient wheat cultivar Jing-411 and iron-inefficient cultivar Sanshumai-3 by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out with the probes of ATPr binding transporter (ABC) cDNA selected from DDRT-PCR products of the cultivar Jing-411 . The results demonstrated that its expression were suppressed under iron-deficiency stress.

关 键 词：小麦 根 铁素营养 MRNA 差别显示 DDRT-PCR分析 基因表达 铁胁迫 

分 类 号：S512.101[农业科学—作物学]