CpG DNA对鸡传染性喉气管炎病毒DNA疫苗免疫效果的影响  

Contribution of CpG DNA to the Immune Effect Induced by DNA Vaccines Against Infectious Laryngotracheitis Virus

作　　者：孟松树[1] 李曦[1] 智海东[1] 王云峰[1] 王柳[1] 童光志[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《中国农业科学》2001年第3期342-344,共3页Scientia Agricultura Sinica

基　　金：中华农业科教人才基金;中国农业科学院跨世纪人才基金

摘　　要：将分别构建的含有鸡传染性喉气管炎病毒（ＩＬＴＶ）王岗株ｇＢｇＣ和ｇＤ基因的重组真核表达质粒及ＣＰＧＤＮＡ佐剂分组肌肉注射 ＳＰＦ鸡，检测了免疫后的抗体水平，并观测了攻毒后的免疫保护效果。实验结果显示，ＣｐＧＤＮＡ佐剂和ＤＮＡ疫苗联合免疫后的抗体水平比单一使用ＤＮＡ疫苗的要高，而佐剂组的发病率、死亡率均低于非佐剂组，保护率则高于非佐剂组。这表明 ＣｐＧ ＤＮＡ佐剂增强了 ＩＬＴＶ ＤＮＡ疫苗的免疫效果。Chickens of different groups were immunized by intramuscular injection of eukaryotic expression recombinant plasmids containing gB. gC and gD genes of infectious laryngotracheitis virus (ILTV) Wanggang strain (DNA vaccines), the above DNA vaccines used in two groups of chickens were mixed with CpG DNA extracted from E. coli . as adjuvants. Specific antibody was detected after immunization and immune effects were observed after challenge. Results showed that the antibody titer detected in chickens immunized with DNA vaccine and CpG DNA were higher than those of chickens without use of CpG DNA, and both morbidity and mortality of chickens with use of CpG DNA were lower than those of chickens without use of CpG DNA. The above results indicated that the immune effect of DNA vaccine against ILTV was enhanced by CpG DNA as adjuvants.

关 键 词：传染性喉气管炎病毒 DNA疫苗 CPG-DNA 免疫效果 鸡 

分 类 号：S858.31[农业科学—临床兽医学]