杨树冰核活性细菌溃疡病血清学的检测技术  

Serology Detection Techniques for Poplar INA Bacterial Canker Disease

作　　者：张景华 柳京花[2] 董爱荣[2] 何秉章[2] 项存悌[2] 朱虹[2] 王传伟 林海波 姜国义 潘淑英 苏贵林 

机构地区：[1]黑龙江省阿城市林业局,阿城市150030 [2]东北林业大学 [3]黑龙江省肇东市林业局 [4]黑龙江省大庆市林业局

出　　处：《东北林业大学学报》2001年第3期128-133,共6页Journal of Northeast Forestry University

基　　金：国家"九五"科技攻关子专题

摘　　要：用从感病杨树上分离纯化得到的４种冰核活性细菌：成团泛氏菌［Ｐａｎｔｏｅａ　ａｇｇｌｏｍｅｒａｎｓ（Ｅ．ｈｅｒｂｉｃｏｌａ）］、菠萝泛氏菌［Ｐ．ａｎａｎａｓ（Ｅａｎａｎａｓ）］、大黄欧父氏菌［Ｅｒｗｉｎｉａ　ｒｈａｐｏｎｔｉｃｉ］和丁香假单胞菌致病变种［Ｐｓｅｕｄｏｍｏｎａｓ　ｓｙｒｉｎｇａｅ　ｐｖ　ｓｙｒｉｎｇａｅ］作为抗原。分别免疫家兔。获得较高滴度的专化抗血清．经试管沉淀试验测得滴度分别为：１／１０２４、１／２０４８、１／１０２４、１／１０２４；专化性反应率为９５．８％．在检测技术研究中．进行了试管沉淀法、玻片凝集法、环状界面凝集法和对流免疫电泳４种试验。通过比较证明、环状界面凝集法用于检测带菌苗木最为方便和可靠、对已知病原菌的检出率达９８％以上。Four species of ice nucleation active bacteria purified from the infected poplar were used as antigens for immunizing rabbits respectively, and high titer products of antiserums were obtained. According to the test tube precipitation tests. the titers reached l/1024. 1/2048. l/1024 and l/1024 separately. and the rate of specific reaction was 95. 8%. Test tube precipitation test. glass sheet coagulation test. ring-shaped boundary surface test and convection immunity electrophouesis test were applied to the detection experiments. and the result showed that the third method was the most convenient and reliable for the detection of the known INA bacteria in the poplar samples, and the accuracy of this method reached 98% or higher.

关 键 词：杨树 冰核活性细菌 溃疡病 血清学检测 

分 类 号：S763.1[农业科学—森林保护学] S763.721.1[农业科学—林学]