棉疫病菌90kD胞外蛋白激发子生物活性与稳定性研究  

BIOACTIVITY AND STABILITY OF 90 kD EXTRACELLULAR PROTEIN ELICITOR FROM Phytophthora boehmeriae

作　　者：张正光[1] 王源超[1] 郑小波[1] 

机构地区：[1]南京农业大学植保系

出　　处：《植物病理学报》2001年第3期213-218,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金 (39970 478);教育部跨世纪优秀人才培养计划基金资助

摘　　要：对棉疫病菌 (Phytophthora boehmeriae Saw.) 90 k D胞外蛋白激发子的生物活性与稳定性进行了研究。用不同浓度的激发子处理烟叶测定其诱发过敏反应的有效浓度 ,结果是所测定的0 .5～ 10 0 nmol/ L各浓度均可诱发过敏反应 ,但 10 0 pmol/ L不能诱发过敏反应 ,表明该激发子诱导烟草产生过敏反应的最低有效浓度在 10 0 pmol/ L～ 0 .5 nmol/ L之间。该激发子可诱导不同烟草 (Nico-tiana tabacum L .)品种产生过敏反应 ,但不能诱导辣椒 (Capsicum annuum L .)和茄子 (Solanum me-longena L.)等茄科作物及棉花 (Gossypium arboreum L inn.)发生过敏反应 ,初步表明该激发子诱导植物发生过敏反应具有一定的专化性。以 10 nmol/ L激发子溶液处理烟叶后分别于第 0、2 4、4 8和 72 h接种烟草疫霉 (Phytophthora nicotianae) ,结果证明该激发子可以诱导烟草产生抗性反应 ,其抗性以处理后立即接种烟草疫霉为最强 ,接种后 4 8h防效达 6 8%,以后随时间的延长逐渐减弱。激发子分别经 p H值 2～ 14的水溶液 2 5℃处理 30 min,或分别经 4、2 5、6 0和 10 0℃处理 5 min仍能诱发过敏反应 ,但是经蛋白酶 K处理后不能诱发过敏反应。表明该激发子对酸、碱和温度不敏感 ,但对蛋白酶 K敏感。Bioactivity and stability of purified 90 kD extracellular elicitor protein from Phytophthora boehmeriae were studied. The elicitor could induce hypersensitive responses (HR) on tobacco leaves at different doses ranging from 0.5 nmol/L to 100 nmol/L but not at 100 pmol/L, which indicated that the minimal efficient dosage of inducing HR on tobacco was between 100 pmol/L and 0.5 nmol/L. The elicitor could also induce HR on other cultivars of Nicotiana tabacum butnotonotherSolanaceae (eggplant and pepper)oroncotton( Gossypium arboreum ), which suggested that this 90 kD elicitor was host-specific. When the 90 kD elicitor was infiltrated into the tobacco leaves, it could induce the systemic acquired resistance (10%~68%) to P. nicotianae that was inoculated on the tobacco stem. The highest level of induced resistance was acquired when tobacco stems were inoculated immediately after its leaves being treated with elicitor at different pH (pH2 pH14) for 30 minutes or at different temperature (4℃, 25℃, 60℃, 100℃) for 5 minutes, the elicitor could maintain its activity of inducing HR. However,the treatment with proteinase K could cause the loss of its ability of inducing HR in tobacco. Results showed that the elicitor was not sensitive to acid, alkali or temperature, but was sensitive to proteinase K.

关 键 词：棉疫病菌 90kD蛋白激发子 生物活性 稳定性 

分 类 号：S432.2[农业科学—植物病理学]