草莓主栽品种再生和转化的研究  

Regeneration and Transformation in Vitro of the Strawberry Varieties

作　　者：张志宏[1] 吴禄平[1] 代红艳[1] 王国英[2] 赵天永[2] 毕晓颖[1] 杜国栋[1] 

机构地区：[1]沈阳农业大学园艺系,沈阳110161 [2]中国农业大学生物技术国家重点实验室,北京100094

出　　处：《园艺学报》2001年第3期189-193,I001,共6页Acta Horticulturae Sinica

摘　　要：建立草莓主栽品种高效、稳定的离体再生体系和遗传转化体系 ,获得了转基因植株。‘弗吉尼亚’和‘森嘎拉’的叶片离体再生芽频率达到 10 0 %。试管苗叶片与农杆菌菌株EHA10 5共培养 3d。共培养后的叶片在附加卡那霉素 4 0mg/L的再生培养基上选择培养 4周后 ,外植体再生出转化芽 ,弗吉尼亚的转化芽再生频率可达 6.8%。采用组织化学染色法对随机选取的 10个GUS基因转化植株进行基因表达测定 ,结果 5个植株强烈表达GUS活性。转bar基因植株在附加除草剂草丁膦 10mg/L的培养基上能够正常分化 ,在田间对草丁膦表现出强烈抗性。转基因植株开花。An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed,and transgenic plants were obtained.Bud regeneration percentage of leaf pieces of strawberry varieties‘Tudla’and‘Senga Sengana’reached 100%.Leaf pieces from in vitro grown plants‘Tudla’were cocultivated for 3 days with Agrobacte rium tumefaciens EHA105.After the cocultivated leaf pieces were incubated on regeneration medium containing 40mg/L kanamycin for 4 weeks,the transformed buds appeared on the explants.The regeneration percentage of transformation buds of strawberry variety‘Tudla’reached 6.8%.The result of histochemical staining of GUS gene showed,of the 10 independent plants transformed with GUS gene,5 expressed GUS activity strongly.The plants transformed with bar gene were able to differentiation on the medium containing glufosinate 10mg/L,and expressed strongly resistance to glufosinate on field.The transgenic plants flowered and fruited normally.

关 键 词：草莓 再生 转化 抗除草剂 品种 遗传改良 

分 类 号：S668.4[农业科学—果树学]