小鼠胚胎肢体器官培养模型的建立  

Establishment of fetal limb bud culture model of mouse

作　　者：余增丽[1] 李云[1] 

机构地区：[1]华西医科大学公共卫生学院,成都610041

出　　处：《卫生研究》2001年第4期217-218,共2页Journal of Hygiene Research

基　　金：国家自然科学基金 (No.3 960 0 12 2 )

摘　　要：为建立一套能简便、客观研究单因素对骨形成影响的方法 ,应用自制浸没式一次通气旋转装置对孕龄 16天的小鼠肢体器官进行体外培养 ,采用X ray摄片及组织学切片技术分析骨发育情况。结果显示 ,经过 6天培养后 ,骨密度增加 ,骨组织呈活跃增殖 ,分化相 ,骨小梁增多 ,成骨区明显。提示胚胎肢体器官能在体外培养中存活并继续生长、发育 。In order to study the development of bone and the effect of each single factor on the metabolism of bone during gestation age, a self made rotating apparatus was designed. The limb bud of fetal mouse at the 16th day of gestation was examined with the techniques of X roentgenography and histological slices. Comparing with the uncultued control limbs, the test limb cultured for six days showed longer bone length and higher bone density. The cultured bone cells showed active differentiation, proliferation and increased bone trabecula by histological examination. It is concluded that the cultured bone continued developing and differentiating, which confirming the feasibility and reliability of the method.

关 键 词：器官培养 胚胎肢体 骨骼发育 实验研究 

分 类 号：Q132.8[生物学—普通生物学] Q954.48