有氧运动改善高脂血症分子机理的研究Ⅲ.运动上调饮食性高胆固醇血症大鼠肝脏LCAT和apoAI基因表达  

Study on the Molecular Mechanism for Favorable Effects of Aerobic Exercise on Hyperlipidemia Ⅲ. Aerobic Exercise Up-regulated the Hepatic Expression of LCAT and apoAI mRNA in Diet-induced Hyperlipidemic Rats

作　　者：张娜[1] 赵斐[1] 张勇[1] 

机构地区：[1]天津体育学院运动医学研究所,天津300381

出　　处：《中国运动医学杂志》2001年第3期232-235,255,共5页Chinese Journal of Sports Medicine

基　　金：天津市自然科学基金资助(项目号983608011;003606211)

摘　　要：目的：以ＲＴ－ＰＣＲ技术测定和分析高脂高胆固醇膳食和有氧运动干预对高胆固醇血症大鼠肝脏卵磷脂胆固醇酯酰转移酶（ＬＣＡＴ）和载脂蛋白 ＡＩ（ａｐｏＡＩ）基因表达的影响，探讨有氧运动对胆固醇逆向转运（ＲＣＴ）通路影响的分子机理。方法：雄性ＳＤ大鼠４０只，随机分为：１）８周普通膳食对照组（ＮＳ，ｎ＝１０），２） ８周高脂膳食对照组（ＨＳ，ｎ＝１０），３）８周普通膳食＋运动组（ＮＥ，ｎ＝１０）和４）８周高脂膳食＋运动组（ＨＥ，ｎ＝１０）。建立高脂高胆固醇膳食诱导ＳＤ大鼠高脂血症的实验动物模型和有氧运动训练模型，用 ＲＴ－ＰＣＲ方法测定肝脏ＬＣＡＴ ｍＲＮＡ和ａｐｏＡＩ ｍＲＮＡ表达量。结果：（１）ＮＥ组大鼠肝脏ＬＣＡＴ ｍＲＮＡ的表达较ＮＳ组显著增高（Ｐ＜０．０５）；（２）ＨＳ组ＬＣＡＴ和ａｐｏＡＩ ｍＲＮＡ表达较ＮＳ组均显著降低（Ｐ＜０．０１）；（３）ＨＥ组ＬＣＡＴ和ａｐｏＡＩ ｍＲＮＡ较 ＨＳ组显著升高（Ｐ＜０．０１），且 ＬＣＡＴ ｍＲＮＡ与 ＮＳ组相比无显著差异，但是ａｐｏＡＩ ｍＲＮＡ的表达仍显著低于ＮＳ组（Ｐ＜０．０１）；（４）肝脏ａｐｏＡＩ ｍＲＮＡ的表达量与血清ＨＤＬ－Ｃ的水平无显著相关；在ＮＥ、ＨＳ和ＨＥ组中肝?Objective: In order to explore the molecular mechanism of the effect of exercise on reverse cholesterol transport (RCT, we observed the effect of exercise on hepatic expression of lecithin cholesterol acyltransferase (LCAT) and apoAI mRNA by RT-PCR. Methods: SD rats were divided into four groups: 1) normal diet for 8 weeks (NS); 2) high -fat-high-cholesterol (HFC) diet for 8weeks (HS); 3) normal diet and aerobic exercise for 8weeks(NE); 4) HFC diet and aerobic exercise for 8weeks. Our study used RT-PCR determining the effect of aerobic swimming exercise on hepatic levels Of LCAT and apoA I mRNA in hyperlipidemic rats. Results: The hepatic levels of LCAT mRNA in NE group are higher than NS group (P＜0.05); The LCAT and apoAI mRNA in HS group are lower than NS group (P＜0.01); The hepatic mRNA levels of LCAT and apoAI of HE group were higher than HS group (P＜0.01). At the same time, it was discovered that there was relationship between the expression of hepatic LCAT mRNA in NE, HS and HE group. Conclusions: HFC diet can down-regulate the hepatic transcription of LCAT and apoAI gene in rats. Swimming training might protect against the atherogenic effects of HFC diet by up-regulate the hepatic transcription of LCAT and apoAI gene, and the expression of LCAT are closely related with the serum levels of HDL-C during the exercise treatment. Thus, these molecular regulating might be responsible for the availability of reverse cholesterol transport in hyperlipidemia.

关 键 词：有氧运动 高脂血症 LCAT APOAI mRNA 胆固醇逆向转运 基因表达 

分 类 号：R87[医药卫生—运动医学]