用亲和层析法纯化牛精浆蛋白  

Purification of Bovine Seminal Plasma Proteins from Bovine Seminal Plasma by Affinity Chromatography

作　　者：赵为[1] 陈菲[1] 刘倩[1] 刘莹[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学教研室,沈阳110001

出　　处：《中国医科大学学报》2001年第4期256-257,263,共3页Journal of China Medical University

基　　金：973国家基础研究重点课题 ;19990 5 5 90 0 2

摘　　要：目的 :从牛精浆中纯化牛精浆 (BSP)蛋白。方法 :利用盐析及亲和层析等方法对BSP蛋白进行纯化。结果 :用Gelatin Sephorase 4B亲和胶纯化出一个蛋白峰 ,经电泳及氨基酸测序证实为BSP A1/ A2蛋白。 结论 :用市售Gelatin Sephorase 4B亲和胶可方便的纯化出BSP A1/ A2蛋白。Objective: Our aim was to purify bovine seminal plasma (BSP) proteins from bovine seminal plasma. Methods: We purified the BSP protein by precipitation and affinity chromatography. Results: We got a protein peak through the Gelatin Sephorase 4B gel and it was identified as BSP A1/ A2 by SDS PAGE and amino acid sequencing. Conclusion: The BSP A1/ A2 protein can be purified by Gelatin Sephorase 4B affinity gel.

关 键 词：纯化 牛精浆蛋白 亲和层析 电泳 

分 类 号：R392－33[医药卫生—免疫学]