骨髓基质细胞体内外成骨的实验研究  

Bone formation by bone marrow stromal cell using tissue engineering methods in vitro and in vivo

作　　者：金丹[1] 裴国献[1] 王前[1] 魏宽海[1] 

机构地区：[1]第一军医大学附属南方医院创伤骨科,广州市510515

出　　处：《中华显微外科杂志》2001年第3期195-197,共3页Chinese Journal of Microsurgery

基　　金：国家重点基础研究发展规划 (973项目 ) (G1 990 5430 9);广东省自然科学基金项目 (990 4 1 4

摘　　要：目的　探讨骨髓基质细胞 (Bone marrow stromal cell,BMSc)向成骨细胞转化的条件 ,利用骨髓基质细胞构建组织工程化骨组织。　方法　采用地塞米松、β-甘油磷酸钠诱导体外培养的兔骨髓基质细胞 ,通过相差显微镜观察和碱性磷酸酶检测其向成骨细胞转化的能力。将骨髓基质细胞与生物活性玻璃陶瓷 (Bioactive glass ceramic,BGC)复合后植入兔自体肌袋内 ,观察成骨过程。　结果　在适当诱导条件下 ,骨髓基质细胞可向成骨细胞分化 ,在体内外表现出明确的成骨能力 ,地塞米松、β-甘油磷酸钠起着重要的作用。　结论　骨髓基质细胞是骨组织工程的良好细胞来源 。Objective To study the osteogenic capability of bone marrow stromal cell (BMSc) using tissue engineering methods Methods The osteogenic potential in vitro of cultured BMSc in a conditional medium were examined by phase contrast microscopy,histochemistry stains technique The BMSc were cultured in combination with bioactive glass ceramic (BGC) materials Then the composite were implanted into the skeletal muscle beds in rabbits All implants were exmined by gross observation and histological examination Results The BMSc showed a similar property to those of osteoblasts and could synthesized mineralized new bone tissue in vitro New bone tissue can be observed in the composites of the BMSc and BGC materials Conclusions New bone tissue can be formed by tissue engineering methods

关 键 词：骨髓 成骨作用 基质细胞 实验研究 细胞培养 

分 类 号：R318[医药卫生—生物医学工程]