不同盐的左氧氟沙星体外抗乙型肝炎病毒的作用及机理的研究  

The inhibitory effect of levofloxacin of various salts on hepatitis B virus in vitro

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作  者:陆海英[1] 王勤环[1] 曾争[1] 傅希贤[1] 于敏[1] 席宏丽[1] 张乃临[1] 公维国[1] 张国庆[1] 刘丹[1] 

机构地区:[1]北京大学第一医院感染疾病科,100034

出  处:《北京医学》2001年第4期228-230,共3页Beijing Medical Journal

摘  要:目的 研究不同盐的左氧氟沙星体外抗 HBV的作用及其机理。方法 以 HBs Ag、HBe Ag及细胞存活率为指标 ,评价药物体外抗 HBV效果 ;检测 HBV DNA、RNA的拷贝数 ,结合 Southern杂交 ,推测其体外抗HBV的作用机理。结果 磺酸左氧氟沙星对 HBs Ag和 HBe Ag的 ID5 0 分别为 63.1μg/m l及 81.3μg/ml,CD5 0 为182 .0 μg/ml,TI>2 ;对细胞内、外 HBV DNA及细胞内 HBV RNA均有剂量依赖性抑制作用 (P<0 .0 5 ) ;Southern杂交显示 ,其对细胞内 HBV DNA及复制中间体有抑制作用 ,对 sc DNA有部分抑制作用。结论 磺酸左氧氟沙星有抗 HBV作用 ,作用位点在 HBV DNA及 scObjective Levofloxacins of various salts were studied for its effect and mechanism on hepatitis B in vitro.Methods HBsAg?HBeAg and the survival cell rate were used to evaluate the effect of the drug on HBV,the mechanism of the drug against HBV was speculated according to the copies of extracellular HBV DNA intracellalar HBV RNA and DNA combined with the result of Southern blot hypbridization.Results HBsAg and HBeAg ID 50 of levofloxacin sulpharate were 63.1μg/ml and 81.3μg/ml respectively,CD 50 was 182μg/ml,TI>2;it could have dose dependent inhibitory effect on extracellular HBV DNA intracelluar HBV RNA and DNA ( P <0.05).The result of Southern blot hybridization showed that it could block HBV DNA and its replicative intermediates,partially inhibit HBV scDNA.Conclusions Levofloxacin sulphurate could block the replication of HBV through inhibiting HBV DNA and scDNA.

关 键 词:Southern吸印杂交 左氧氟沙星 定量PCR 抗乙型肝炎病毒 乙型肝炎 

分 类 号:R512.62[医药卫生—内科学]

 

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