肺炎克雷伯杆菌感染引起大鼠小气道炎症的免疫学特征  

Immunological Characterization of Small Airway Inflammation of Rats Induced by Klebsiella Pneumoniae

作　　者：许浒[1] 熊密[2] 吴焕明[2] 刘绍春[2] 郝春荣[2] 赵时宇[2] 黄庆华[3] 车东媛[2] 

机构地区：[1]镇江医学院病理学教研室,镇江212001 [2]同济医科大学病理学教研室,武汉430030 [3]同济医科大学微生物学教研室,武汉430030

出　　处：《上海免疫学杂志》2001年第4期229-231,共3页Shanghai Journal of Immunology

基　　金："九五"国家医学科技攻关基金资助项目 (96 90 6 0 16 ) ;镇江医学院博士科研启动基金资助项目 (2 3 6 2 0 6 )

摘　　要：本文观察了肺炎克雷伯杆菌气道感染引起大鼠小气道炎症细胞和肺组织中TNF α含量动态变化。结果显示 ,肺炎克雷伯杆菌感染第 1天至第 1周 ,小气道壁中炎症细胞以中性粒细胞增多为主。感染第 2周至第 16周 ,小气道壁T淋巴细胞较对照组明显增多 (P <0 0 1) ,其阳性细胞百分率范围在 39%～ 67%。感染第 8周和第 16周 ,小气道壁CD4/CD8比值下降 ,分别为 0 5 7和 0 41,同时间对照组为 0 75和 0 78。树突状细胞 (S 10 0阳性细胞 )分布在小气道上皮 ,固有膜和粘膜下层。感染第 1周至第 8周 ,肺组织中TNF α含量明显升高 (P <0 0 1)。因此 ,在克雷伯杆菌感染引起的小气道炎症中 ,小气道壁T淋巴细胞 ,尤其CD8T细胞浸润值得重视。同时 ,肺组织中TNF α含量增高可能是T淋巴细胞在肺内积聚和数量增多机制之一。In this study,dynamic changes in the small airway inflammatory cells of rats and the TNF α contents in the lung tissues were examined Experimental results demonstrated that the neutrophils predominated in the small airway inflammatory cells from the first day to the first week after Klebsiella pneumoniae infection,and from the second week to 16th week,T lymphocytes in the small airway epithelium,mucosa and submucosa markedly increased in comparison with the controls with a positive cell range of 39% to 67% Up to 8th and 16th week,the CD4/CD8 ratios dropped in the small airway,appearing to be 0 57 and 0 41 respectively (0 75 and 0 78 in the control groups) One week after infection,the TNF α contents in the lung tissues increased and kept a higher levels until 8th week Thus,it is important to consider the significance of T lymphocyte infiltrations in small airway inflammation induced by Klebsiella pneumoniae ,especially the infiltration of CD8 + cells Meanwhile,the elevation of TNF α contents in the lung tissued might be one of the mechanisms involving the accumulation and increase in numbers of T lymphocytes in lungs

关 键 词：小气道炎症 细菌感染 淋巴细胞 免疫学 

分 类 号：R563.9[医药卫生—呼吸系统] R378.996[医药卫生—内科学]