体外血管钙化模型中骨钙素的分泌和X型胶原的表达  

Osteocalcin secretion and the expression of type X collagen mRNA in an in vitro model of vascular calcification

作　　者：崔晓暄[1] 王士雯[2] 齐鹏[2] 

机构地区：[1]北京空军总医院,100036 [2]北京解放军总医院老年心血管病研究所

出　　处：《心肺血管病杂志》2001年第3期176-178,共3页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然科学基金资助 (基金编号 :39770 86 9)

摘　　要：目的 :研究 2 5 羟基胆固醇对体外培养的主动脉中膜细胞钙化的促进作用 ,以及在此过程中骨钙素的分泌和X型胶原mRNA的表达与钙化的关系 ,初步探讨主动脉钙化的机制。方法 :采用外殖块法分离培养家兔主动脉中膜细胞 ,进行vonkossa染色以显示钙化 ,显微分光光度法检测细胞内外不溶性钙。平衡法1 2 5 I放免测定培养上清骨钙素的含量。RT PCR方法检测X型胶原mRNA的表达。结果 :2 5 羟基胆固醇 (实验组 )培养的传代细胞可见多个细胞结节 ,且细胞结节vonkossa染色阳性 ;而对照组无细胞结节形成 ,vonkossa染色阴性。前者每孔不溶性钙沉积量 ,以及培养上清中骨钙素含量明显高于后者。且实验组X型胶原mRNA的表达为阳性 ,而对照组为阴性。结论 :2 5 羟基胆固醇可促进主动脉中膜细胞发生钙化。主动脉中膜在体外钙化过程中与成骨细胞相似 ,骨钙素分泌增多且有X型胶原mRNA的表达 。Objective:To investigate the stimulation to in vitro calcification of aorta by 25 hydroxycholesterol,and the relationship of the calcification with the secretion of osteocalcin and expression of type X collagen mRNA.Method:Aortic medial cells were obtained by explantation from rabbits von Kossa staining was performed to show the in vitro calcification.Insoluble calcium of cellular layer was determined by biochemical microspectrophotometric method.And osteocalcin in the media was analyzed with balanced radioimmunoassay.RT PCR was used to determine the expression of type X collagen mRNA.Result:Passaged cells showed negative von Kossa after 28 days.The cells treated with 25 hydroxycholesterol appeared many cell nodules with positive von Kossa staining,in which much more insoluble calcium and supernatant osteocalcin were detected.Type X collagen mRNA was detected in cells treated with 25 hydroxycholesterol but not in untreated cells.Conclusion:25 hydroxycholesterol can accelerate the in vitro calcification of aortic media.Aortic medial cells secrete more osteocalcin and express type X collagen mRNA during calcification,which is similar to osteoblast,suggesting arterial calcification may share part of the same mechanism with the calcification of the bone. [

关 键 词：X型胶原 骨钙素 25-羟基胆固醇 动脉血管壁 钙化 

分 类 号：R543.5[医药卫生—心血管疾病]