不同流动剪切力调节大鼠成骨样细胞增殖的体外研究  

The Effect of Fluid Shear Stress on the Proliferation of Rat Primary Osteoblast like Cells in vitro

作　　者：陈伟辉[1] 乔鞠[1] 罗颂椒[1] 李声伟[1] 田卫东[1] 李芃[1] 

机构地区：[1]华西医科大学口腔医学院,成都610041

出　　处：《华西医科大学学报》2001年第2期232-234,239,共4页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助!(批准号 3 960 0 167)

摘　　要：目的　从细胞水平探索机械应力刺激调节骨改建的机制 ,并探讨促进骨改建的最适生理应力值。方法　对体外培养的大鼠成骨样细胞施加一系列不同大小的流动剪切力 ,利用流式细胞技术衡量其细胞的分裂增殖能力。结果　大鼠成骨样细胞受到流动剪切力后 ,细胞与流动方向一致的长轴被拉长 ,细胞沿流动方向排列。随着剪切力的增加 ,成骨样细胞的增殖指数 (PI)不断增加 ,当剪切力为 12 dyn/cm2 时 ,PI值与对照组相比有显著性差异 (P<0 .0 5 )。随剪切力的继续增加 ,PI值开始下降 ,当剪切力大于 14dyn/cm2 时 ,PI值与对照组相比显著下降 (P <0 .0 1)。结论　机械应力对骨改建的调节作用与成骨细胞受到应力诱导产生的流动剪切力的刺激有关 ,低剪切力对细胞的增殖影响不大 ,中等大小的剪切力 (12 dyn/cm2 )明显刺激细胞的增殖 ,高剪切力将抑制细胞的增殖。Objective To inquire into the cellular mechanism by which mechanical stress regulates bone remodeling and make an attempt at establishing the most suitable physiological stress to stimulate bone formation. Methods The isolated rat primary osteoblast like cells were exposed to fluid shear stress 2, 4, 8, 12, 14, 17and 19 dyn/cm 2 for 60 min respectively in the flow chamber. The ability of proliferation of cells was studied by flow cytometry. Results After exposed to fluid shear stress, the cells rearranged and the cells' axes were elongated following the flow direction. The proliferation indexes (PI) of the cells went up with the gradual increase of shear stress. When fluid stress reached 12 dyn/cm 2, the PI of cells increased significantly ( P <0.05), compared with the control. But the PI of cells began to decrease when the fluid stress continued to increase, and when fluid stress reached 14 dyn/cm 2, the PI of cells decreased significantly ( P <0.01), compared with the control. Conclusion The mechanism by which mechanical stress regulates bone remodeling is related to the proliferation of the osteoblast cells stimulated by stress induced fluid flow. Low shear stress has no marked influence on the proliferation of cells. Medium shear stress (12dyn/cm 2) can stimulate cells significantly. Major shear stress (14dyn/cm 2) can inhibit cells significantly.

关 键 词：骨改建 成骨样细胞 流动剪切力 细胞增殖 大鼠 

分 类 号：R318.01[医药卫生—生物医学工程]