端粒重复序列扩增法结合微孔板杂交法定量检测端粒酶活性  

Quantitative detection of telomerase activity by telomeric repeat amplification protocol with microtiter hybridization analysis

作　　者：温淑娟[1] 况二胜[1] 黄镇华[1] 

机构地区：[1]第一军医大学南方医院医学基因技术中心,广东广州510515

出　　处：《第一军医大学学报》2001年第6期457-458,共2页Journal of First Military Medical University

摘　　要：目的探讨定量检测端粒酶活性的非放射性检测方法。方法将端粒重复序列扩增测定法（ＴＲＡＰ）与微孔板杂交 相结合建立了非放射性检测方法，对３例正常肝组织和４例肝癌患者治疗前后的肝癌组织、瘤旁组织、肝癌凋亡组织 进行检测。结果肝癌组织具有比较高的端粒酶活性，而正常肝组织和瘤旁组织无此酶活性，肝癌凋亡组织端粒酶活性 显著降低。结论　该定量分析方法简单、灵敏、特异性强，可以比较准确地反映组织的端粒酶活性状态。Objective: To explore a non-radioactive method for quantitatively measuring telomerase activity. Methods A nonradioactive method for quantitative measurement of telomerase activity was established by combining telomeric repeat amplification protocol(TRAP) and microtiter hybridization, and was subsequently applied in the determination of telomerase in the speciemens of normal and carcinomous hepatic tissues, tissues adjacent to the neoplasm and the apoptotic neoplastic tissues obtained from 4 patients with hepatocarcinoma before and after therapy. Results High levels of telomerase expression was observed in hepatic carcinomous tissues, whieh was absent in the tissues adjacent to the neoplasm and significantly decreased in the apoptotic neoplastic tissues. Conclusion This non-radioactive quantitative method is convenient and specific. in measuring telomerase activity.

关 键 词：端粒 重复序列扩增测定法 微孔板杂交 末端转移酶 肝肿瘤 

分 类 号：R346[医药卫生—基础医学]