反相高效液相色谱法同时测定气喘片中4组份的含量  

Determination of four components in anti-asthma tablets by RP-HPLC

作　　者：安富荣[1] 王平全[1] 戈升荣[1] 费艳秋[1] 袁静[1] 

机构地区：[1]上海第二医科大学仁济医院临床药学研究室,上海200001

出　　处：《中国医院药学杂志》2001年第6期336-338,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：测定气喘片中茶碱、苯巴比妥、盐酸麻黄碱和盐酸苯海拉明的含量。方法：采用RP-HPLC法,在Hypersil CN柱上，以乙腈-水相（1%三乙胺，用冰醋酸调pH6.5） 为流动相，检测波长为215 nm。结果：本法可同时测定4组分的含量。茶碱、苯巴比妥、盐酸麻黄碱和盐酸苯海拉明分别在16～12 0，6～42，5～35 μg· ml-1浓度范围内，峰面积与其浓度呈良好线性关系，平均回 收率依次为99.4%(RSD为0.91%),99.3%(RSD为1.16%),97.7%( RSD为1.26%)和98.3%(RSD为1.14%)。结论：方法切实可行，简便，快速，准确。：OBJECTIVE To separate and determine four constituents including theophylline,phenobarbita l,ephedrine hydrochloride and diphenhydramine hydrochloride in anti-asthma tab lets. METHODS An RP-HPLC was used.Using Hypersil CN（CPS2）column(4.6 mm×250 mm,5 μm), mobile phase was consisted of acetonitrile and water (1%triethylamine，adjust w ith glacial acdtic acid to a pH of 6.5)(35∶65).The detection wavelength was 215 nm. RESULTS The separation and determination of four constituents could be accomplished simu ltaneously.The calibration curves were linear in the range of 16～120 μg· ml -1 for theophylline，6～42 μg·ml-1 for phenobarbital, 5～35 μg· ml -1 for ephedrine hydrochloride and diphenhydramine hydrochloride.The aver age recoveries of theophylline, phenobarbital,ephedrine hydrochloride and diphen hydramine hydrochloride were 99.4%(RSD=0.91%),99.3%(RSD=1. 16%),97.7%(RSD=1.26%) and 98.3%(RSD=1.14%),respectivel y. CONCLUSIONS The method is simple, quick and accurate for the quality control.

关 键 词：反相高效液相色谱法 茶碱 苯巴比妥 盐酸麻黄碱 盐酸苯海拉明 气喘片 

分 类 号：R974.3[医药卫生—药品] R927.2[医药卫生—药学]