酶联免疫吸附实验法测定缺血大鼠电针后皮质神经营养因子含量的变化  

Measurement of Changes of Brain-derived Neurotrophic Factor Content in Rat Ischemic Cerebral Cortex after Electroacupuncture with Enzyme Linked Immunosorbent Assay

作　　者：陈英辉[1] 黄显奋[1] 

机构地区：[1]上海医科大学神经生物教研室,上海200032

出　　处：《针刺研究》2001年第2期93-96,共4页Acupuncture Research

基　　金：国家自然科学基金资助项目 (No.39730 5 10 )

摘　　要：应用酶联免疫吸附实验 (ELISA)法测定缺血大鼠电针后脑内脑源性神经营养因子(BDNF)含量的变化。实验分正常组、缺血再灌组和缺血再灌 +电针组 ,大脑中动脉阻塞 (MCAo)致局灶缺血 90min ,缺血后立即给予电针 ,取“水沟”和“百会”穴 ,强度 3.5mA ,频率 1 0 0Hz,时间1hr。再灌 8hr后取缺血侧皮质 ,ELISA法测定。结果正常组、缺血再灌组和缺血再灌 +电针组脑内BDNF含量分别为 1 4.2 0± 3.1 4、1 8.75± 2 .6 3和 2 3.75± 3.0 2 (ng/ g) ,缺血 +电针组BDNF含量多于缺血组 (P <0 .0 5 )。Objective: To observe the effect of electroacupuncture (EA) on brain derived neurotrophic factor(BDNF) content of the cerebral cortex in rats with cerebral ischemia. Methods: 18 Wistar rats were randomly and evenly divided into normal control, ischemia and EA+ischemia groups. The middle cerebral artery was occluded for 90 min to induce focal ischemia. After cerebral ischemia, EA (3.5 mA, 100 Hz) was applied to “Shuigou” (GV 26) and “Baihui” (GV 20) for one hour. After 8 hr reperfusion, the ischemic cortex was sampled and the BDNF content determined with enzyme linked immunosorbent assay (ELISA). Results: It was found that BDNF contents in the infarction region of the cerebral cortex in control, EA and EA ischemia groups were 14.20±3.14, 18.75±2.63 and 23.75 ±3.02 ng/g respectively. Conclusion: EA could increase the synthesis and release of BDNF in the brain after cerebral ischemia and reperfusion.

关 键 词：脑缺血 电针 脑源性神经营养因子 

分 类 号：R246.6[医药卫生—针灸推拿学] R245.97[医药卫生—中医临床基础]