胰岛素样生长因子Ⅰ受体在宫内发育迟缓胎鼠中的表达研究  

Studies on Insulin like Growth Factor Ⅰ Receptor in Fetal Rats with Intrauterine Growth Retardation

作　　者：杨凡[1] 姚裕家[1] 李炜如[1] 陈永秀[1] 唐军 曾懿[1] 白波 

机构地区：[1]华西医科大学附属第二医院儿科,成都610041 [2]广州市花都区医院儿科

出　　处：《华西医科大学学报》2001年第3期402-404,共3页Journal of West China University of Medical Sciences

摘　　要：目的　探讨宫内发育迟缓 (IU GR)时胰岛素样生长因子 受体 (IGF- R)表达的变化及其在组织和细胞中的定位。方法　将 2 1只 SD孕鼠随机分为实验组 12只和对照组 9只。钳夹实验组孕鼠双侧子宫动、静脉 2 0分钟 ,建立宫内发育迟缓动物模型。对照组仅作开腹和关腹术。两组均于孕 2 2天剖宫取出胎鼠 ,采用免疫组化法观察孕晚期胎鼠肝脏和肺组织中 IGF- R的表达。结果　实验组胎鼠的体重、身长及胎盘、肝、肺组织重量较对照组明显降低 (P<0 .0 5 ) ;实验组胎鼠肝脏 IGF- R表达面积比较对照组明显增加 ,平均灰度明显降低 (P<0 .0 1) ;实验组胎鼠肺组织 IGF- R表达面积比也较对照组明显增加 (P<0 .0 5 ) ,但平均灰度则无显著性差异 (P>0 .0 5 )。结论　宫内发育迟缓时 IGF- R在肝脏和肺组织的表达增加 ,可能是机体对 IGF-Objective This study was conducted to detect the localization of the insulin like growth factor Ⅰ receptor (IGF ⅠR) in the cells and tissues in the rats with intrauterine growth retardation (IUGR) and normal growth rats. Methods 21 pregnant rats were randomly divided into the experimental group (IUGR) ( n =12) and the control group ( n =9). Animal models for IUGR were established by clamping the uterine vasculature of pregnant rats for 20 minutes on day 14 of gestation. The control group rats were subjected to Sham operation. On day 22 of gestation, fetal rats were delivered by cesarean section. Immunohistochemical assay was performed to detect the IGF ⅠR expression on liver and lung using specific polyclonal antibodies to rat IGF ⅠR. Results In the experimental group, the IUGR rats' body weight, height, and the weight of their liver, lung and the placenta were significantly lower than those in the control group. In the IUGR rats' liver, the area ratio of IGF ⅠR was increased and the average gray level was decreased, compared with those in the control group. In the IUGR rats' lung, the area ratio of IGF ⅠR was increased, compared with that in the control group, but no difference of average gray level was observed between the two groups. Conclusion The increased expression of IGF ⅠR in rats with IUGR, may be due to the decrease of IGF Ⅰand the compensative mechanism of the body.

关 键 词：宫内发育迟缓 胰岛素样生长因子I受体 免疫组织化学 胎鼠 IUGR 

分 类 号：R714.51[医药卫生—妇产科学]