rIFNα-2b对小鼠脾脏NK细胞活性的影响  

The Effect of rIFNα-2b on Mice NK Cell Activity

作　　者：张平[1] 魏大鹏[1] 王蓉[1] 赵宗容[1] 王霞[1] 蔡美英[1] 

机构地区：[1]华西医科大学基础医学院免疫学教研室,成都610041

出　　处：《华西医科大学学报》2001年第3期408-408,427,共2页Journal of West China University of Medical Sciences

摘　　要：目的　研究重组干扰素 α- 2 b(r IFNα- 2 b)对小鼠脾脏 NK细胞杀伤活性的影响 ,了解该干扰素的生物学活性。方法　采用皮下给药的体内实验及 3 H- Td R掺入抑制法检测小鼠脾脏 NK细胞活性。结果　 r IFNα- 2 b能显著提高小鼠脾脏 NK细胞杀伤活性 ,且呈剂量依赖性 ,其高、中、低剂量各组的 NK活性分别为 65 .19± 17.5 6、44 .19± 14.30、35 .46± 17.49,与空白对照及阴性对照均有显著性差异。结论　 r IFNα- 2 b能提高 NK细胞活性 ,并有剂量依赖性。Objective This animal experiment was made to study the effect of the recombined interferon α 2b(rIFNα 2b) on natural killer cells activity of mice spleen in vivo, and by way of this,to test the activity of rIFNα 2b. Methods BALB/c mice were given rIFNα 2b subcutaneously. The inhibiting 3H TdR intaking method was used to test the NK activity of spleen cells of BALB/c mice. Results The NK cell activity levels of the high dose group, the mid dose group and the low dose group were 65.19±17.56, 44.19±14.30 and 35.46±17.49,respectively. Conclusion rIFN a 2b can enhance NK cells activity observably and its effect is dose dependent.

关 键 词：重组干扰素Α-2B 小鼠 NK活性 脾脏 

分 类 号：R392.11[医药卫生—免疫学]