CD42a定量检测血小板  

Counting Platelets by Flow Cytometry with Platelet specific Antibody CD42a

作　　者：彭黎明[1] 江虹[1] 杨慧[2] 朱新勤[1] 

机构地区：[1]华西医科大学附属第一医院检验科,成都610041 [2]云南省第一人民医院病理科

出　　处：《华西医科大学学报》2001年第3期474-478,共5页Journal of West China University of Medical Sciences

摘　　要：目的　探讨血小板 (PL T)计数的参考方法。方法　以 FITC标记的 CD42 a单克隆抗体特异结合PL T膜糖蛋白 Ib- Ix(GPIb- Ix) ,用流式细胞仪术 (FCM)检测 76例全血 FITC-抗 CD42 a- GPIb/ Ix定量 PL T。用FCM测得 PL T/ RBC,乘以经准确校正的血细胞分析仪所测同一标本的 RBC值 ,得 PL T结果 (× 10 9/ L)。同时 ,将FCM所测 PL T结果与 SE- 95 0 0、NE- 15 0 0、CD- 160 0及手工法测定结果相比较。结果　 FCM批内重复性高值 (5 0 1× 10 9/ L)及中值 (184× 10 9/ L)标本的变异系数 (CV) <4% ,低值 (2 3× 10 9/ L)标本 CV=11.432 % ;批间重复性 CV为 10 .792 % ,总重复性 CV<5 % ;PL T在 2 0× 10 9/ L～ 70 0× 10 9/ L 范围内线性良好 (r=0 .9975 ,P<0 .0 0 1)。测定结果在标本采集后 6小时内稳定 ,携带污染率为 0 .87%。FCM与 SE- 95 0 0、NE- 15 0 0、CD- 160 0、手工法测定 PL T结果间有良好相关性 (r>0 .975 )。结论　 FCM测定 PL T- CD42 a定量 PL T特异、准确、精密 ,可望成为 PL T测定的参考方法。Objective To investigate a reference method for platelet (PLT) count. Methods 76 samples of daily whole blood of high,normal and low values in patients were collected and analyzed by flow cytometry (FCM) with CD42a monoclonal antibody that could combine specifically with the platelet membrane glycoprotein Ib Ix (GPIb Ix). PLT counts were analyzed by multiplying PLT/RBC ratio from FCM with the RBC that was counted by a calibrated blood analyzer. Meanwhile, the results of PLT from FCM were compared with that from blood analyzers SE 9500, NE 1500, CD 1600 or manual count. Results The within replicate coefficient of variance(CV) of FCM was less than 4% for a high count(501×10 9/L) and middle count(184×10 9/L), the CV of low count(23×10 9/L) was 11.432%,the between replicate CV was 10.792%, and the overall reproducibility was less than 5%. Linearity was demonstrated well( r =0.9975 P <0.001) when the PLT counts were 20×10 9/L ~700×10 9/L. PLT count was stable at room temperature within 6hr. The carry over was 0.87%. The results between FCM and SE 9500,NE 1500,CD 1600 or manual count was comparable ( r >0.975, P <0.001). Conclusion These findings confirm that FCM for PLT count is specific, accurate and precise and could be considered as a candidate of reference method for PLT count.

关 键 词：血小板计数 CD42a 流式细胞术 参考方法 

分 类 号：R446.111[医药卫生—诊断学]