纳屈酮对大鼠急性出血坏死性胰腺炎致胰性脑组织损害的治疗作用  

THERAPEUTIC EFFECT OF NALTREXONE ON PANCREATIC ENCEPHALOPATHY INDUCED BY ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS IN RATS

作　　者：赵海平[1] 欧阳晓晖[1] 刘淑萍[2] 孔广忠[1] 杨成旺[1] 寿乃延[1] 

机构地区：[1]内蒙古医学院附属第一医院普外科,呼和浩特010050 [2]内蒙古医学院生化教研室

出　　处：《中国普外基础与临床杂志》2001年第3期135-137,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨大鼠急性出血坏死性胰腺炎（ＡＨＮＰ）并发胰性脑组织损害机理及评价纳屈酮治疗效果。方法应用５％牛磺胆酸钠逆行胰胆管注射诱发大鼠ＡＨＮＰ并发脑组织损害模型。实验分为对照组、胰性脑组织损害组（ｐａｎｃｒｅａｔｉｃ ｅｎｃｅｐｈａｌｏｐａｔｈｙ， ＰＥ）及纳屈酮（ＮＴＸ）治疗组，分别于 ６、１２、２４小时检测血浆及脑组织脂质过氧化产物丙二醛（ＭＤＡ）含量、磷脂酶 Ａ２（ＰＬＡ２）活性、氧自由基清除剂超氧化物歧化酶（ＳＯＤ）活性、脑系数及胰腺和脑组织病理学改变。结果与对照组比较，ＰＥ组６、１２、２４小时血浆及脑组织中ＭＤＡ含量及ＰＬＡ２活性升高，ＳＯＤ活性下降；光镜下见ＰＥ组６、１２及２４小时胰腺红细胞渗出、炎性细胞浸润及灶性坏死、脑组织水肿、点状出血和脱髓鞘改变；６小时电镜下见胰腺细胞线粒体肿胀，嵴模糊，粗面内质网扩张；１２小时线粒体破损，粗面内质网脱颗粒；２４小时线粒体及粗面内质网破裂，次级溶酶体出现。 ＮＴＸ治疗组各时相血浆及脑组织 ＭＤＡ含量及 ＰＬＡ２活性较 ＰＥ组低， ＳＯＤ活性却较 ＰＥ组高；且胰腺及脑组织的损害程度减轻。结论大鼠ＡＨＮＰ模型可引发胰性脑组织损害；ＮＴＸ可降低血浆及脑组织氧自由基（ＯＦＲ）?Objective To study the relationship between oxygen free radical and phospholipase A2 and therapeutic effect of naltrexone (NTX) on experimental pancreatic encephalopathy (PE) induced by acute hemorrhagic necrotizing pancreatitis (AHNP) in rats. Methods A model of experimental PE in AHNP was induced by retrograde injection of 5 % sodium taurocholate into the pancreatic duct. The rats were randomly divided into three groups: control group, PE group and NTX group. The plasma and cerebral levels of malondialdenhyde (MDA ), scavengers superoxided ismutase (SOD), and phospholipase A2 (PLA2 ) were determined in both PE and NTX groups. Changes of the pancreatic and cerebral histology were examined by light and electric microscopy. Results In NTX treatment phase, the MDA and PLA2 were significantly fall, while SOD was increased in the plasma and cerebral tissue, the damage to pancreatic and cerebral tissue was emiliorated. Conclusion The experimental model of PE on rats is an ideal one for PE investigation. NTX could decrease oxygen free radicals and PLA2 activity and improve the damage to cerebral and pancreatic tissue. The lethality rate in rats of PE is significantly low after NTX treatment.

关 键 词：急性出血坏死性胰腺炎 胰性脑组织损害 纳屈酮 大鼠 

分 类 号：R657.51[医药卫生—外科学] R741.05[医药卫生—临床医学]