异育银鲫对嗜水气单胞菌灭活菌苗的免疫应答  

Immune Response of Allogynogenetic Crucian Carp( Carassius auratus gibelio ) against Aeromonas hydrophila

作　　者：张秋胜[1] 陈昌福[1] 

机构地区：[1]华中农业大学农业部农业微生物重点实验室,武汉430070

出　　处：《华中农业大学学报》2001年第3期271-274,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金资助项目 (批准号 :39870 5 98)

摘　　要：采用福尔马林灭活的嗜水气单胞菌 (Aeromonashydrophila)菌苗免疫接种异育银鲫 ,通过测定受免鱼血清中凝集抗体效价 ,溶菌酶活性 ,血液中吞噬细胞的吞噬活性 ,杀菌活性并通过鱼体攻毒试验 ,探讨了异育银鲫对福尔马林灭活的嗜水气单胞菌菌苗的免疫应答状况。结果表明 ,接种 2 8d后 ,受免鱼血清中凝集抗体效价和溶菌酶活性均值分别为 1∶18.89和 15 6 .36U/ml,相应的对照组则为 1∶0 .83和 138.6 8U/ml;受免鱼血液中白细胞吞噬百分比和吞噬指数分别为 6 3.38%和 6 .0 2 ,而对照组的分别为 47.95 %和 5 .0 8;免疫组和对照组白细胞的杀菌指数分别为 1.5 9和 1.15 ;受免鱼的免疫保护力达到 73.7%。Allogynogenetic crucian carp( Carassius auratus gibelio )were vaccinated by injecting with the formalin killed A.hydrophila (F?AH).The level of immune response (the agglutinating antibody titres, lysozyme activity in serum,the phagocytic and bactericidal activity of leucocyte in blood) was detected. The immunized group, 28 days after immunization, the serum antibody titre and serum lysozyme activity reached 1∶18∶89 and 156.36 U/ml respectively, while the control group were 1∶0.83 and 138.68 U/ml respectively; The phagocytic percent(PP) and phagocytic index(PI) of leucocyte in blood were 63.38% and 6.02, while the control were 47.95% and 5.08 respectively. The bactericidal index(BI) of leucocyte in blood of vaccinated group was 1.59, while the control group was 1.15. The challenge test of the live pathogen was also carried out.The results showed that vaccination enhanced the immnune response of the fish. The relative percent survival of vaccinated fish was up to 73.7%.

关 键 词：异育银鲫 嗜水气单胞菌 免疫应答 灭活菌苗 

分 类 号：S943.117[农业科学—水产养殖] S941.42[农业科学—水产科学]