光镜对尿中红细胞形态观察的意义  

Significance of RBC morphology in urine by light microscope

作　　者：苏晓林[1] 臧秀娟[1] 项永谦[1] 王力宁[1] 周希静[1] 

机构地区：[1]中国医科大学第一临床学院肾内科,110001

出　　处：《辽宁医学杂志》2001年第4期181-182,共2页Medical Journal of Liaoning

摘　　要：目的　探讨应用细胞活体染色方法在光镜下观察尿中红细胞形态对临床诊断的意义。方法　应用位相差显微镜和应用红细胞活体染色在普通光镜下分别观察了 16 6 1例尿标本 (其中肾小球性血尿 1483例 ,非肾小球性血尿 178例 )尿中红细胞形态并做对比。结果　在 1483例肾小球性血尿中 ,位相差镜的准确率为 99.8% ,而光镜为99.1%。在 178例非肾小球性血尿中 ,位相差镜和光镜的准确率均为 10 0 %。对 16 6 1例尿标本的观察 ,两者总符合率为 99.4%。结论　应用细胞活体染色方法在普通光镜下检查尿中红细胞形态来鉴别肾小球性和非肾小球性血尿的方法与位相差镜下观察的结果一致 ,并且简便、准确性亦高 ,可在基层医疗单位中广泛应用。Objective To study the significance of RBC morphology by light microscope using cell intravital staining. Methods 1 661 specimens with hematuria were detected by phase microscope and light microscope using intravital staining(specimens with nephritic hematuria were 1 483 and nonnephritic hematuria were 178). Results In 1 483 specimens with nephitic hematuria accuracy rate was 99.8% by phase microscope and 99.1% by light microscope.In 178 specimens with nonnephritic hematuria specimens accuracy rate was both 100%.Correspondence rate of two methods(phase and light microscope) was 99.4% in all hematuira specimens. Conclusion There are no significant difference by light microscope and by phase microscope to study the RBC morphology. Differentiation of nephritic and nonnephiric hematuria by light microscope using RBC intravital staining is a simple and highly accuracy method, It may be used widely in basic medical units.

关 键 词：尿 红细胞形态 肾小球肾炎 活体染色 畸形 光镜 

分 类 号：R446.12[医药卫生—诊断学]