1-甲基-4-苯基吡啶离子诱发大鼠小脑颗粒细胞凋亡  

Induction of rat cerebellar granular neuron apoptosis by 1-methyl-4-phenyl pyridinium cation

作　　者：蒲小平[1] 李晓蓉[1] 李慧农 李长龄 

机构地区：[1]北京医科大学药学院药理室,北京100083

出　　处：《中草药》2001年第8期715-718,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展规划项目课题 No.G19980 5 112 4

摘　　要：目的　建立 1-甲基 - 4-苯基吡啶离子 (1- m ethy1- 4- phenyl pyridinium ion,MPP+ )诱导的大鼠小脑颗粒细胞凋亡模型。方法　 MPP+ 处理大鼠小脑颗粒细胞 ,分别用甲基绿 -派诺宁染色 ,DNA凝胶电泳 ,流式细胞术进行检测。结果　浓度为 5 0 μmol/L MPP+使小脑颗粒细胞发生凋亡。结论　以 MPP+为诱导剂建立的大鼠小脑颗粒细胞凋亡模型 ,可用于研究和帕金森病 (PD)有关的细胞凋亡的调控机制和筛选抗Object To establish a rat cerebellar granular neuron (CGN) apoptosis model by 1 methy 4 phenyl pyridinium cation (MPP +) Methods Rat CGN were treated with MPP + and the resulting cell morphology examined by methyl green pyronine staining, agarose gel electrophorsis of DNA and flow cytometry Results MPP + at the concentration of 50 μ mol/L can induce CGNs apoptosis of the established model Conclusion The CGNs apoptosis model induced by MPP + can be used for the study on regulatory mechanism of cell apoptosis and the screening of antiparkinsonian drugs

关 键 词：1-甲基-4-苯基吡啶离子 MPP^+ 小脑颗粒细胞 细胞凋亡 帕金森病 

分 类 号：R971[医药卫生—药品] R742.5[医药卫生—药学]