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作 者:邓学梅[1] 李俊英[1] 李宁[2] 吴常信[1]
机构地区:[1]中国农业大学动物科技学院,北京100094 [2]中国农业大学生物技术国家重点实验室,北京100094
出 处:《Acta Genetica Sinica》2001年第9期801-807,共7页
基 金:国家自然科学基金资助(39725022); 北京市自然科学基金资助(5981001)~~
摘 要:采用F-2设计,以丝羽乌骨鸡(C系)为一亲本,分别与农大褐蛋鸡(B系),及法国明星肉 鸡(A系)进行正反交,产生了包含A × C,C × A;B × C,C × B 4个杂交组合的F2代群体,建 立了可用于定位产肉及产蛋等性状QTL的资源群体。基于这一F-2群体,对体重及日增重等 性状表型值进行分析,结果显示,亲本间均值差异显著,分离群体变异大,这组性状间有中等 或较高的相关,各性状潜在的QTL座位数不超过10,所建资源家系能够满足QTL定位所需的 样本数量,达到了预期的效果。A three-generation of chicken population is being constructed for mapping multi-economical trait loci where three extremely divergent breeds, including a broiler breed(A), a layer breed(B) and white-silk chicken(C) were employed for reciprocal cross. Four cross combinations of A♂× C♀, C♂ ×A♀; B♂ ×C♀, C♂ ×B♀ were performed with 277 individuals in total parental population. According to the statistic analysis for growth trait, difference between parental lines is significant, F2 generation attains great variation. Correlations among body weight and daily gain traits are significant and relative coefficients are moderate or high. The number at QTL loci is expected to be no more than 10, and based on the theoretical computation the sample size of this resource population is sufficient for QTL mapping.
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