鸭卵泡及精巢中卵泡抑素和抑制素/活化素β_B亚基mRNA表达的研究  

Expression Pattern of mRNA for Follistatin and Inhibin/Activinβ_B- subunit During Follicular and Testicular Development in Duck

作　　者：傅衍[1] 牛冬[1] 阮晖[2] 余旭平[1] 陈功[1] 何国庆[2] 杨培新[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江大学农业工程与食品科学学院,杭州310029

出　　处：《Acta Genetica Sinica》2001年第9期808-815,共8页

基　　金：国家自然科学基金(批准号:39900106)资助~~

摘　　要：卵泡抑素是与抑制素／活化素功能密切相关的一种糖蛋白激素，采用定量竞争ＲＴ－ＰＣＲ 技术对鸭各级卵泡及成熟与未成熟精巢中的卵泡抑素和抑制素／活化素βＢ亚基的ｍＲＮＡ表达 丰度进行了研究，发现在上述组织中均有此两基因ｍＲＮＡ的表达，且只在小卵泡中表达较丰 富。卵泡抑素在小黄卵泡（ＳＹＦ）中表达量最高，以其ｍＲＮＡ的平均相对丰度为１．００，则Ｆ１（最 大卵泡）、Ｆ２、Ｆ３、Ｆ４、Ｆ５、Ｆ６～８、ＬＷＦ（大白卵泡）、ＴＩ（未成熟精巢）和ＴＭ（成熟精巢）中卵泡抑素 ｍＲＮＡ的平均相对含量分别为０．０１１±０．００４、０．０１９±０．００６、０．０２１±０．００９、０．０２８±０．００７、 ０．０７５ ± ０．０２３、０．１５ ± ０．０７２、０．２９ ± ０．０６８、０．０３７ ± ０．０１１和０．０１２±０．００４。βＢ亚基也在小黄卵 泡中表达量最高，以其ｍＲＮＡ的平均相对丰度为１．００，则 Ｆ１、Ｆ２、Ｆ３、Ｆ４、Ｆ５、Ｆ６－８、ＬＷＦ、ＴＩ和ＴＭ 中βＢ亚基ｍＲＮＡ的平均相对含量分别为：０．００９ ± ０．００３、０．０１３±０．００５、０．０１９ ± ０．００７、０．０２３ ± ０．００６、０．２９±０．０８４、０．８４±０．Follistatin and inhibin / activin were closely related glycoprotein hormones. The quantitative competitive RT-PCR was used to investigate the expression of follistatin and inhibin / activin βB-subunit mRNA in the developing ovarian follicles, immature and mature testes. The results revealed all samples showed the expression of mRNA for the two proteins, and the expression is more abundantly in the small follicles than in the large preovulatory follicles. Competitive RT-PCR revealed that the expression of follistatin is the highest in small yellow follicles (SYF), the mean relative content for the F1, F2， F3，F4, F5, F6-8, LWF (large white follicle), TI(immature testes), and TM(mature testes) was 0.011± 0.004, 0.0 19± 0.006, 0.021± 0.009, 0.028 ± 0.007, 0.075 + 0 023 0.15± 0.072，029 + 0.068, 0.037± 0.011, and 0.012 ±0.004,respectively, compared to a mean relative content of 1.00 for the SYF。 The highest level of inhibin/ activinβB mRNA was also found in the SYF, the mean relative content for the F1, F2, F3, F4, F5, F6-8, LWF, TI, and TM was 0. 009± 0.003, 0.013±0.005, 0.019± 0.007, 0.023± 0.006, 0.29± 0.084, 0. 84± 0.093, 0.031± 0.008, 0.38±0.072,and 0.046± 0.013, respectively, compared to a mean relative content of 1.00 for the SYF. Our data suggested that the expression pattern of mRNA for follistatin and inhibin/ activinβB- subunit was quite similar during follicular and testicular development The great co-expression of mRNA for the two proteins in small follicles indicated that activin B(βB - βB) availability was tightly regulated by follistatin, and the two proteins might both play important roles in early follicular development.

关 键 词：定量竞争RT-PCR 卵泡抑素 抑制素 活化素βB亚基 卵泡 精巢 鸭 禽类 

分 类 号：S834.2[农业科学—畜牧学]