不同品种猪肌肉生长抑制素基因单核苷酸多态性分析  

Analysis on Single Nucleotide Polymorphisms of Porcine Myostatin Gene in Different Breeds

作　　者：姜运良[1] 李宁[1] 吴常信[2] 杜立新[3] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094 [2]中国农业大学动物科技学院,北京100094 [3]山东农业大学动物生物工程研究室,泰安271018

出　　处：《Acta Genetica Sinica》2001年第9期840-845,共6页

基　　金：国家自然科学基金重点项目(39730360); 国家重点基础研究发展规划项目(G20000161)~~

摘　　要：用ＰＣＲ－ＲＦＬＰｓ和ＰＣＲ－ＳＳＣＰ分析方法，对“双肌臀”大白猪、大白猪、长白猪、杜洛克、汉普夏、皮特兰、二花脸、东北民猪、湖北白猪和部分杂交猪等不同品种猪肌肉生长抑制素基因３＇编码区、５＇调控区及内含子１区３个单核苷酸多态性位点（ＳＮＰｓ）进行了分析。结果表明，３＇编码区的ＳＮＰ发生的频率较低，在２７４头猪中未检出突变纯合体。对５＇调控区的ＳＮＰ，引进猪种（大白猪、长白猪、杜洛克、汉普夏和皮特兰）及其杂交猪以等位基因Ｔ为主，二花睑和湖北白猪则以等位基因Ａ为主，均偏离Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡状态（Ｐ＜０．０１）。东北民猪的３种基因型近乎相等，处于Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡状态。对内含子１区的 ＳＮＰ，大白猪及其与长白猪的杂交猪等位基因Ｇ占优势，二花脸和湖北白猪则以等位基因Ａ为主，均偏离Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡状态（Ｐ＜０．０１）；东北民猪和大二猪的等位基因Ｇ和Ａ近乎相等，处于Ｈａｒｄｙ－Ｗ ｅｉｎｂｅｒｇ平衡状态。“双肌臀”大白猪在５＇调控区和内含子１区这两个位点的Ａ等位基因稍高于普通大白猪．５＇调控区和内含子１区ＳＮＰｓ所产生的等位基因表现出连锁遗传现象。By PCR-RFLPs and PCR-SSCP approach, three single nucleotide polymorphisms (SNPs) of porcine myostatin gene (MSTN) were analyzed in different breeds including doubled-muscled' Yorkshire, Yorkshire, Landrace, Hamshire, Duroc, Piteran, Erhualian, Min, Hubei White and some hybrids. The three SNPs were located in the 3＇ encoding region, 5＇ promoter region and intronl region respectively. For the SNP in the 3＇ encoding region, which was caused by C→T transition, the mutation frequency was relatively low: no TT genotype was detected in 274 individuals of different breeds. For the SNP in the 5＇ promoter region, 560 pigs were investigated. The allele T dominates in the imported lean-type pig breeds such as Yorkshire, Landrace, Duroc, Hampshire, Piteran and hybrid, however, in Erhualian and Hubei White pigs, the allele A was in majority. Polymorphism showed the similar pattern for the SNP in intron 1 region. G was the dominant allele in Yorkshire, Landrace and their hybrids, while in Erhualian and Hubei White pigs the frequency of A was much higher. Obviously they were not in Hardy-Weinberg equilibrium state. For Min and Yorkshire× Erhualian pigs, they were in Hardy-Weinberg equilibrium state for the SNPs in the 5＇ promoter region and (or) intron 1 region. The frequency for the A alleles of SNPs in the 5＇ promoter region and intron 1 region was higher for 'double-muscled' Yorkshire than for Yorkshire and linkage for these two mutation sites was also observed.

关 键 词：肌肉生长抑制素基因 单核苷酸多态性 猪 品种 

分 类 号：S828.1[农业科学—畜牧学]