TGF-β在小鼠胎盘中的表达  

Expression of TGF-β in the Mouse Placenta

作　　者：高飞[1] 马捷[1] 傅国强[1] 魏鹏[1] 冯强[1] 刘以训[1] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室,北京100080

出　　处：《Zoological Research》2001年第4期269-274,共6页动物学研究（英文）

基　　金：WHO/洛氏基金联合研究项目;"973"项目;"攀登"项目;"创新工程"项目

摘　　要：利用原位杂交和免疫组化的方法对小鼠胎盘中的TGF - β (transformgrowthfactor β)mRNA和蛋白质进行检测 ,结果显示 :在妊娠第 7 5～ 9 5天 ,TGF - β主要在子宫蜕膜中表达 ,而且表达逐渐增强。说明这一时期TGF - β的作用主要是控制滋养层细胞的侵入 ,这种控制是通过对PA (plasminogeninactivotor)和MMP(matrixmetalloproteinase)两类主要的细胞外蛋白水解酶的抑制作用来实现的。到妊娠 10 5天时 ,滋养层巨细胞明显表达TGF - βmRNA和蛋白质 ,这与其功能的转换是一致的。因为此时的滋养层巨细胞体积变大 ,停止增殖 ,其功能也从侵入型向内分泌型转换。海绵滋养层细胞从第 9 5天开始表达TGF - βmRNA ,而TGF - β抗原从第 10 5天开始出现 ,第 11 5～ 12 5天 ,TGF - βmRNA的表达达到最高 ,此时的TGF - β的作用是调控胎儿血管的形成。另外 ,TGF - β对免疫系统有强烈的抑制作用 ,妊娠中后期海绵滋养层细胞可能通过大量表达TGF- β以调节局部免疫 ,避免母体对胎儿的免疫排斥。Expression of TGF-β mRNA and protein in mouse placenta was detected using in situ hybridization and immunohistochemistry.The results showed that TGF-β mRNA and protein were mainly detected in the deciduas between D7.5 and D9.5 of pregnancy and the expression gradually decreased.The data indicate that TGF-β might be involved in regulation of trophoblast invasion during this period and may act through inhibiting the activity of two types of extracellular proteinase,PAs and MMPs.On D10.5 of pregnancy,trophoblast giant cells expressed TGF-β mRNA and protein.This was consistent with the fact that the trophoblast giant cells stoped to proliferate and invade at this time.On D9.5,spongiotrophoblast cells began to express TGF-β mRNA,while its protein was detected in these cells on D10.5,and the expression of TGF-β mRNA reached the peak level between D11.5 and D12.5 indicating it maybe also involved in regulation of the formation of fetal vasculature during this period.Furthermore,the maternal tolerance to fetal semi-allograft was partly attributed to the expression of TGF-β,because TGF-β could inhibit the maternal immune system.

关 键 词：转化生长因子 小鼠 胎盘 滋养层细胞 海绵滋养层细胞 滋养层巨细胞 

分 类 号：Q954.4[生物学—动物学]