Preliminary Isolation of Hormone Stimulating Muscle Protein Synthesis in Thoracic Ganglia from the Prawn, Macrobrachium rosenbergii  

罗氏沼虾胸神经节中促肌肉蛋白质合成激素的初步分离(英文)

作　　者：廖家遗[1] 孙继贤[1] 

机构地区：[1]中山大学生命科学学院,广州510275

出　　处：《Zoological Research》2001年第4期275-278,共4页动物学研究（英文）

基　　金：NationalNaturalScienceFoundationofChina ( 39670 10 3)

摘　　要：The results of the previous studies seem to show the crustaceans can secrete growth hormone,which regulate their growth.However,more evidences are needed to determine it.So the following research was conducted.The extract of thoracic ganglia (TGE) of adult Macrobrachium rosenbergii was applied to a Sephadex G-50 column,the resulting second peak was further isolated by HPLC,and 9 fractions were obtained.Among the fractions only the second fraction significantly increased the protein synthesis of the abdomen muscle of the juvenile M.rosenbergii that was injected TGE.The increasing effects were related to the protein concentration of the injecting fractions.The molecular weight of the substance in the second fraction isolated by HPLC was below 3.4?kDa.The results indicate that the thoracic ganglia of M.rosenbergii could secrete a peptide hormone,which stimulates the muscle protein synthesis of prawn,and the hormone might be the growth hormone of the prawn.罗氏沼虾 (Macrobrachiumrosenbergii)成虾的胸神经节匀浆液 ,经SephadexG - 5 0柱层析所得的第 2个峰 ,再经HPLC分离得到 9个组分。把第 2个组分的溶液注入罗氏沼虾幼虾体内 ,其肌肉蛋白质合成明显增加 (P <0 0 1) ,这种促进蛋白质合成的能力随该溶液的蛋白质浓度的增加而增强。结果表明罗氏沼虾胸神经节能分泌促进虾肌肉生长的激素。该激素的分子量在 3 4kDa以下 ,可能是虾的生长激素。

关 键 词：Macrobrachium rosenbergii Thoracic ganglion Protein synthesis Growth hormone ISOLATION 

分 类 号：Q57[生物学—生物化学]