5-HT受体亚型mRNAs在大鼠背根神经节和三叉神经节的表达—原位杂交组织化学法研究  

EXPRESSION OF 5-HT RECEPTOR SUBTYPE mRNAs IN THE DORSAL ROOT GANGLION AND TRIGEMINAL GANGLION OF THE RAT-IN SITU HYBRIDIZATION HISTOCHEMICAL STUDY

作　　者：武胜昔[1] 朱敏[1] 王亚云[1] 王文[1] 李云庆[1] 

机构地区：[1]第四军医大学基础医学部解剖学教研室,梁銶琚脑研究中心西安710032

出　　处：《神经解剖学杂志》2001年第2期131-135,T025,共6页Chinese Journal of Neuroanatomy

基　　金：国家杰出青年科学基金 (No.3 96 2 5 0 11);国家重点基础研究规划<脑功能和脑重大疾病的基础研究>基金 (G19990 5 40 0 0 )资助项目

摘　　要：应用原位杂交组织化学技术观察了大鼠背根节和三叉神经节内 5 -HT2 、5 -HT3 、5 -HT4、5 -HT5 、5 -HT6 和 5 -HT7受体亚型 m RNAs的表达。结果显示 :在背根节和三叉神经节中均观察到了 5 -HT2 A、5 -HT3 、5 -HT4和 5 -HT7受体亚型 m RNAs阳性细胞 ;两种神经节内上述受体亚型 m RNAs阳性细胞的百分比不同。 5 -HT3 受体亚型 m RNA阳性细胞的百分比最高 ,分别为 3 0 .4% (背根节 )和 3 2 .8% (三叉神经节 )。约 17.2 %和 14 .6 %的背根节和三叉神经节细胞分别呈 5 -HT2 A受体 m RNA阳性 ;而 5 -HT4和 5 -HT7受体亚型 m RNAs阳性细胞在此二节中的百分比分别为 14 .9%、2 4.7%和 11.2 %、9.8%。各受体亚型 m RNA阳性细胞均以中、小型节细胞为主。在各神经节内均未观察到 5 -HT2 B、5 -HT2 C、5 -HT5 和 5 -HT6 受体 m RNA的表达。上述结果提示 5 -HT2 A、5 -HT3 、5 -HT4和 5In situ hybridization histochemical technique was used to observe the expression of 5 HT 2 7 receptor subtype mRNAs in the rat dorsal root ganglion (DRG) and trigeminal ganglion (TG). The results showed that positive cells for 5 HT 2A , 5 HT 3, 5 HT 4 and 5 HT 7 receptor subtype mRNAs were observed in both DRG and TG. The percentage of positive cells for each 5 HT receptor subtype mRNA to the total number of the counted cells in the two ganglia was different. The highest percentage of positive cells was present for 5 HT 3 receptor subtype mRNA in these two ganglia, 30.4% in DRG and 32.8% in TG?About 17.2% and 14.6% of DRG and TG cells were 5 HT 2A receptor subtype mRNA positive cells; while the percentages of 5 HT 4 and 5 HT 7 receptor subtype mRNAs positive cells in DRG and TG were 14.9%, 24.7% and 11.2%, 9.8%, respectively. The positive signals were mainly confined to a subpopulation of small and medium sized cells. No expression of 5 HT 2B , 5 HT 2C , 5 HT 3, 5 HT 5 and 5 HT 6 receptor subtype mRNAs was detected in DRG and TG in the present study. The present results suggested that 5 HT 2A , 5 H 3, 5 HT 4 and 5 HT 7 receptor subtypes might play important roles in peripheral nociceptive signaling. (Figure 1 on plate 25)

关 键 词：5-HT受体亚型 反转录PCR 原位杂交组织化学 伤害性感受 感觉神经节 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]