鸡胚脊髓背角中神经营养活性物质的初步分离和检测  

Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos

作　　者：刘耀波[1] 薛庆善[2] 肖渝平[3] 王先荣[4] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]中山医科大学生理学教研室,广州510089 [3]中山医科大学生物学教研室,广州510089 [4]兰州医学院组织学与胚胎学教研室,兰州730000

出　　处：《生理学报》2001年第4期321-324,共4页Acta Physiologica Sinica

基　　金：theNationalNatureScienceFoundationofChina (No 39470 2 44 )

摘　　要：从 9d龄鸡胚脊髓背、腹角组织提取液中 ,用SephadexG 75凝胶层析法分离出DⅠ和DⅡ ,VⅠ和VⅡ各组蛋白质组分 ,体外培养检测各组分对鸡胚背根节的神经营养活性。结果表明 ,DⅡ组分具有明显的促背根节神经突起生长的作用 ,而DⅠ组分无此作用 ;经SDS PAGE电泳检测 ,DⅡ组分中蛋白质的分子量范围是 61～ 15kD。而腹角的分离组分VⅠ和VⅡ对背根神经节神经突起的生长并无明显作用。Extracts of spinal dorsal horn and ventral horn of chicken embryos hatched for 9 days were separated by Sephadex G 75 gel filtration, and different chromatographic fractions of DⅠ and DⅡ, VⅠ and VⅡ were obtained. The neurite outgrowth promoting effects of the various fractions were assayed by the dorsal root ganglion (DRG) culture in vitro. It was shown that DⅡ, but not DⅠ, fraction of the dorsal horn extract exhibited a significant neurite outgrowth promoting effect on DRG. By means of SDS PAGE, the molecular weight of DⅡ fraction ranged from 61 to 15 kD. VⅠ and VⅡ fraction of ventral horn showed no obvious effect on neurit outgrowth of DRG in vitro .

关 键 词：脊髓背角 神经营养活性 背根神经节 胚胎发育 

分 类 号：Q954.4[生物学—动物学]