两株钝顶螺旋藻紫外诱变株的特征  

CHARACTERISTICS OF TWO SPIRULINA PLATENSIS MUTANTS INDUCED BY ULTRAVIOLET

作　　者：李建宏[1] 郑卫[1] 倪霞[1] 翁永萍[1] 潘欣[1] 浩云涛[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097

出　　处：《水生生物学报》2001年第5期486-490,共5页Acta Hydrobiologica Sinica

基　　金：江苏省教委自然科学基金资助(98KJD180002)

摘　　要：采用紫外诱变的方法筛选获得了两株优良的稳定钝顶螺旋藻突变株M1 - 3和M5-1。与出发株相比 ,M 5- 1较粗大 ,M1 - 3较细 ,但很长 ,藻体螺旋数超过 40 ;两株突变株的生长速度和光合放氧速率均有显著提高 ;M1 - 3的藻蓝蛋白含量高于出发藻株 2 0 2 % ;突变株的长碳连不饱和脂肪酸含量高于出发藻株 ,总脂中M 1 - 3含花生四烯酸 (2 0∶4) 4 93 %、M5- 1含EPA(2 0∶5) 2 49%。两株突变株对NH+4和Zn2 +的抗性也发生了改变。Spirulina platensis was induced by ultraviolet irradiation as a mutagen. Two good mutants,M1-3 and M5-1,were selected. M5-1 was bigger than PS (Paprent Strain),and M1-3 was thinner and much longer with its spiral number of one filament being over 40;The growth and photosynthetic O 2 evolution rates of two mutants were higher than those of PS obviously. Phycocyanin content in M1-3 was 20.2% higher than those of PS. The two mutants had more long carbon chain polyunsaturated fatty acids. The M1-3 contained 4.93% arachidonic acid(20∶4),and M5-1 contained 2.49% EPA (20∶5) in the total fat. The antitoxic abilities of two mutants to toxic ions,NH + 4 and Zn 2+ were different from PS also.

关 键 词：钝顶螺旋藻 紫外诱变 藻蓝蛋白 不饱和脂肪酸 

分 类 号：Q949.22[生物学—植物学] S968.4[农业科学—水产养殖]