IGF-ImRNA在不同年龄鲤表达的差异和LHRH-A对IGF-ImRNA表达的影响  

DIFFERENTIAL EXPRESSION AND EFFECT OF LHRH A ON EXPRESSION OF IGF I mRNA IN JUVENILE AND ADULT COMMON CARP(CYPRINUS CARPIO)

作　　者：华益民[1] 林浩然[1] 

机构地区：[1]中山大学生命科学学院,广州510275

出　　处：《水生生物学报》2001年第5期498-502,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(批准号:39570099);中山大学基础学科前沿课题基金

摘　　要：利用RNA酶保护法对 7月龄性未成熟幼鲤和 2龄性成熟鲤组织胰岛素样生长因子 -I(IGF -I)mRNA的表达水平进行测定 ,结果表明成鱼肝和肾脏组织IGF -ImRNA的丰度显著高于幼鱼。对鲤成鱼和幼鱼腹腔注射促性腺激素释放激素类似物 (LHRH -A ,D -Ala6-Pro9-NEt-LHRH)使血清生长激素 (GH)水平和肝组织IGF -ImRNA水平都显著升高 ,而成鱼生殖腺IGF -ImRNA的丰度比对照组显著增加。研究结果提示鲤在不同发育阶段肝组织IGF -ImRNA的表达存在差别 ,其中 2龄成鱼大于 7月龄幼鱼 ;LHRH -A可能通过刺激垂体GH的释放间接促进肝组织IGF -ImRNA的表达 ,亦可能通过某种未知途径刺激生殖腺IGF -ImRNA的表达。IGF I mRNA expression of 7-month old juvenile and 2-year old adult common carp were determined by RNase Protection Assay. Results showed that the hepatic IGF I mRNA level of adult fish was significantly higher than that of 7-month old juvenile fish; while in other tissues there was no significant difference between the two fish groups except that kidney IGF I mRNA level of adult fish was higher than that of juvenile fish. After twice i. p. injections of LHRH A, the levels of serum GH and hepatic IGF I mRNA in fish of both ages increased greatly, and the IGF I mRNA level of gonadal tissue in adult fish increased when compared with controls. No changes in amount of IGF I mRNA in other tissues were observed after injections of LHRH A. It is suggested that the level of hepatic IGF I mRNA expression changes with different developmental stages; LHRH A may stimulate the production of IGF I by promoting the synthesis and release of pituitary GH; and probably, LHRH A stimulates the IGF I expression in gonads by other mechanism.

关 键 词：鲤 胰岛素样生长因子-I 年龄 LHRH-A 硬骨鱼类 表达 

分 类 号：Q959.4[生物学—动物学] S965.116[农业科学—水产养殖]