力学因素对人红细胞葡萄糖运输和阴离子交换的影响(英文)  

Effect on Glucose Transport and Anion Exchange in Human Erythrocytes by Mechanical Force Factors

作　　者：李月舟[1] 周汉清[1] 彭锋[1] 张志鸿[1] 

机构地区：[1]复旦大学生命科学学院

出　　处：《生物化学与生物物理学报》2001年第5期489-496,共8页

基　　金：国家自然科学基金资助项目 No .39730 15 0和No .30 0 70 2 0 5&&

摘　　要：机械应力在心血管系统的正常生理和病理中都起着重要的作用。实验中观察到当提高红细胞悬浮液的旋转速度时会导致葡萄糖跨膜输入的速率增加。改变溶液渗透压及用使细胞膜曲率变化的药物 (氯丙嗪 )是对红细胞作用的另二种力学因素 ,研究发现它们同样也能对葡萄糖和阴离子的运输有影响。根据运输速率的温度特性给出了这些力学因素作用下葡萄糖、阴离子运输时活化能的变化。活化能的减小和运输速率的增加有很好的对应关系 :活化能减小使膜上运输蛋白在运输过程中的构象变化更为容易。对红细胞血影的内禀荧光淬灭测量表明 ,机械应力是通过影响膜上葡萄糖运输蛋白 (GLUT1)和阴离子交换蛋白 (带 3蛋白 )的构象起作用的。当用抑制剂抑制了阴离子的运输后 ,观察到此时葡萄糖跨膜运输对机械应力的响应发生改变 ,这再次表明在红细胞膜上GLUT1和带The mechanical forces play an important role in both the normal physiology and the pathology of the cardiovascular system. It was observed that an increase in speed of rotary flow of the intact erythrocyte suspension resulted in increase in the rate of glucose entry across the erythrocyte membranes. The effects of osmotic stress and a membrane curvature altering drug, chlorpromazine, on the glucose and anion transport were also shown. The decrease in the activation energy for glucose and anion transport according to Arrhenius plot for temperature dependence of membrane transport meant that thermodynamic barrier for transporter reorientation in the membranes was reduced. The data from the measurement of intrinsic fluorescence quench in ghosts indicated that the conformations of both glucose transport protein (GLUT1) and anion transport protein (band 3) in the erythrocyte membranes were affected by the mechanical force factors. After inhibition of anion transport, a modification of the response of glucose transport by the mechanical force was observed, which also indicates that there is an information linkage between GLUT1 and band 3 in the erythrocyte membranes.

关 键 词：机械应力 红细胞 葡萄糖运输 阴离子交换 

分 类 号：Q689[生物学—生物物理学]