超氧阴离子自由基对大鼠脑皮层神经细胞的损伤作用  

Effects of the Superoxide Anionic Radicals to the Cultured Cerebral Cortex Neuron of Newborn Rat

作　　者：姜招峰[1] 何湘平[2] 刘传缋[2] 杨翰仪[3] 

机构地区：[1]北京联合大学应用文理学院生物化学系,北京100083 [2]军事医学科学院毒物药物研究所,北京100850 [3]吉林大学医学部生物化学教研室,长春130021

出　　处：《生物化学与生物物理学报》2001年第5期552-558,共7页

摘　　要：为了探讨超氧阴离子自由基对神经细胞的影响 ,以产生超氧阴离子自由基系统 (XO/X)作用于原代培养的新生大鼠大脑皮层神经元 ,分析神经细胞的生长状态、蛋白质被氧化修饰的程度、脂质过氧化程度、核DNA损伤程度、亚细胞结构变化以及Cu/ZnSOD基因表达等变化。结果显示 :超氧阴离子自由基可使神经细胞的生长状态不佳 ,ATP酶活性降低为 4.2 2 3μmol/ (mg·min-1) (Pr) ,细胞总羰基含量增加为 2 95 .40 μmol/ g湿重 ,LPO含量增加为 4.87nmol/ g(Pr) ,膜脂流动性下降 (P值为 0 .398) ,核DNA单链断裂增加 (DNA在SCG中的迁移率为 7.35mm) ,亚细胞结构变化 (线粒体肿胀和变性等 )以及SOD基因表达增强 (SOD含量、SOD活性和SODmRNA丰度均增加 )。结果说明超氧阴离子自由基可损伤神经细胞的蛋白质、核酸。The effect of the superoxide anionic radicals to the cultured cerebral cortex neuron of newborn rat was investigated by using xanthine oxidase/xanthine. The results are as follows. The growth of neuron was inhibited; ATPase activity decreased to 4.223 μmol/(mg·min -1 )(Pr); total cellular carbonyl increased to 295.40 μmol/g wet weight; lipid peroxides(LPO) increased to 4.87 nmol/g(Pr); membrane lipid fluidity decreased (P value: 0.398); in nuclear DNA, the single strand breaks(SSB) increased [in the single cell gel assay(SCG), DNA migration was 7.35 mm]; subcellular structures of the neuron changed (mitochondria were swelling, etc.); and SOD gene expression of the neuron increased (the SOD activity and the SOD content increased, SOD mRNA content increased). These suggest that the superoxide anionic radicals could damage proteins, nucleic acids, lipids and subcellular structures in neurons.

关 键 词：超氧阴离子自由基 神经元细胞损伤 CUZN-SOD 脂质 核酸 脑皮层 

分 类 号：Q26[生物学—细胞生物学]