广西眼镜蛇毒磷脂酶A_2初步晶体学分析  

Preliminary Crystallographic Analysis of Phospholipase A_2 from Naja naja kaouthia Lesson Venom

作　　者：王志清[1] 庄茂辛[1] 宋时英[2] 林政炯[2] 舒雨雁[1] 

机构地区：[1]广西医科大学蛇毒研究所,南宁530021 [2]中国科学院生物物理研究所生物大分子国家重点实验室,北京100101

出　　处：《生物化学与生物物理学报》2001年第5期582-584,共3页

基　　金：国家自然科学基金 (No .39970 174);中国科学院重大项目 (No .KJ95 1 A1 6 0 1)资助&&

摘　　要：广西眼镜蛇毒酸性磷脂酶A2 具有抗凝血活性和溶血活性。采用悬滴气相扩散法 ,培养出该酶四方和立方两种晶型的单晶 ,并进行了X射线衍射鉴定。四方晶型的空间群为P432 12或P412 12 ,晶胞参数为a =b =8.797nm ,c=10 .831nm ,每不对称单位中含 3个分子 ;立方晶型的空间群为P2 13 ,晶胞参数为a =b =c =6 .840nm ,其不对称单位含 1个分子。对两种晶型分别收集了 0 .2 8nm分辨率衍射数据。An acidic phospholipase A 2 isolated from the venom of Naja naja kaouthia Lesson in Guangxi exhibits anticoagulative and hemolytic activities. In this work, the enzyme was crystallized by the method of hanging drop vapor diffusion. Two crystal forms were obtained and characterized by Xray diffraction. One of them belonged to space group P4 32 12 or P4 12 12 with unit cell parameters a=b=8.797 nm, c=10.831 nm and there were three molecules per asymmetric unit; the other belonged to space group P2 13 with unit cell parameters a=b=c=6.840 nm and there was one molecule per asymmetric unit. The diffraction data were collected up to 0.28 nm for each crystal form. The crystal properties of Naja naja verom phospholipase A 2 from different geographical regions are compared.

关 键 词：眼镜蛇毒 磷脂酶A2 结晶 晶体特性 

分 类 号：Q516[生物学—生物化学]