自主神经系统参与低氧下的免疫调节作用  

THE AUTONOMIC NERVOUS SYSTEM INVOLVED IN REGULATION OF IMMUNE FUNCTION IN RATS DURING HYPOXIA

作　　者：白海波[1] 杜继曾[2] 郑筱祥[1] 

机构地区：[1]浙江大学生命科学与医学工程学系 [2]浙江大学生物科学与技术系,浙江杭州310027

出　　处：《中国应用生理学杂志》2001年第3期224-226,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金 (3 9470 2 77);中国博士后科研基金资助项目

摘　　要：目的 :探讨低氧条件下自主神经系统对大鼠脾淋巴细胞转化的调节作用。方法 :检测减压低氧下外周血中神经递质与脾淋巴细胞转化。结果 :大鼠 5km低氧暴露 2 4h ,脾淋巴细胞对丝裂原反应性下降 ,外周交感神经损毁后则可阻断低氧对此的抑制作用 ;小鼠于真空瓶中 0 .0 7MPa缺氧 10min血浆中去甲肾上腺素 (NE)与肾上腺素 (E)均明显升高 ;大鼠 5km低氧 2 4h ,血浆中乙酰胆碱水平下降 ;体外培养的大鼠脾淋巴细胞中加入不同浓度的乙酰胆碱 ,胸腺嘧啶核苷掺入作用呈浓度依赖性增加。结论 :以上结果提示自主神经系统参与低氧下的免疫调节 ,交感神经系统有免疫抑制作用 。Aim: To study of autonomic nervous system modulation of lymphocyte proliferation during hypoxia. Methods: Rats were exposed to 5 km and 7 km altitude in a hypobaric chamber for 24 h or 48 h, the effect of autonomic nervous system on mitogen induced spleen lymphocyte response to the acute hypoxia was investigated. Results: When rats were exposed to 5 km altitude for 24 h, which inhibited spleen T lymphocyte proliferation induced by concanavalin (Con A), but the action was abolished through peripheral sympathetic destroyed. When mouse were exposed anoxia environment at 0.07 MPa for 10 min, the norepinephrine and epinephrine in plasma were increased markedly. T cells of rats were in vitro incubated simultaneously with ACh showing a concentration dependent enhancement of T cell proliferation. The plasma levels of ACh were decreased after rats were exposed to 5 km for 24 h. Conclusion: Autonomic nervous system was involved in the regulation of immune function in rats during hypoxia.

关 键 词：低氧 脾淋巴细胞转化 乙酰胆碱 交感神经损毁 免疫调节 

分 类 号：R594.3[医药卫生—内科学] R392.1[医药卫生—临床医学]