IL-1受体拮抗剂减轻离体猪肾缺血再灌注损伤的作用及其机制  

THE EXPERIMENTAL STUDY ON PROTECTIVE EFFECTS AND ITS MECHANISMS OF INTERLEUKIN 1 RECEPTOR ANTAGONIST ON ISOLATED SWINE KIDNEY WITH ISCHEMIA/REPERFUSION

作　　者：赵春玲[1] 张春来[1] 李莉华[1] 冯志强[1] 李先华[2] 李达兵[2] 

机构地区：[1]泸州医学院生理教研室 [2]泸州医学院肾功能保护教研室,四川泸州646000

出　　处：《中国应用生理学杂志》2001年第3期247-250,共4页Chinese Journal of Applied Physiology

基　　金：四川省教委基金资助课题 (9713 3 )

摘　　要：目的 :研究IL 1受体拮抗剂 (IL 1ra)对离体猪肾缺血 再灌注 (IR)损伤的作用及机制。方法 :采用离体猪肾自体全血再灌注损伤模型 ,将 32只猪肾随机分为对照组 (n =11)、IR组 (n =11)和IL 1ra组 (n =10 )。供肾猪均电击制动。对照组开腹后 ,即取肾下极作检测。IR组和IL 1ra组分别取再灌注 2h尿及再灌注 2 5h肾组织作检测。结果 :IR组肾皮质中MDA、TNF α、IL 8含量高于对照组 ,而SOD、Na+ K+ ATP酶活力低于对照组 ,差异均具有显著性(P <0 .0 1,以下同 )。IL 1ra组肾皮质中MDA含量和SOD活力分别高于和低于对照组 ,差异均具有显著性 ;同IR组比较 ,IL 1ra组MDA、TNF α、IL 8含量和尿量蛋白含量较低 ,SOD、Na+ K+ ATP酶活力较高。差异均具有显著性。IL 1ra组光、电镜下肾组织结构损伤较IR组轻。结论 :ILAim: To observe the protective effects and approach its mechanisms of interleukin 1 receptor antagonist(IL 1ra) on the isolated swine kidney with ischemia/reperfusion(IR).Methods: Thirty two swine were divided randomly into control group ( n =11), IR group ( n =11) and IL 1ra treatment group (IL 1ra group, n =10) in the study. All swine were electrified. The kidneys were removed and put in ice box immediately for detecting in control group. In IR group and IL 1ra group, reperfusion pressure and flow rate were both observed once every 30 minutes, the urine at the time of reperfusion 2 hours and the renal cortex at the time of reperfusion 2 hours and 30 minutes were taken for detecting.Results: The resistance of perfusion in IL 1ra group was much lower than that in the IR group during reperfusion except start of reperfusion, there was a significant difference between IR group and IL lra group ( P <0.05,under the same). The content of MDA,TNF α and IL 8 in the renal cortex in IR group were higher and the activities of SOD and Na + K + ATPase were lower than those in control group, In IL 1ra group,the content of MDA and the activity of SOD in renal cortex was respectively higher or lower than that in control group. Compared with IR group,the content of MDA and TNF α and IL 8 in the renal tissus and urine protein were lower and the activities of SOD and Na + K + ATPase were higher in IL 1ra group.The serious degeneration and necrosis of renal tissue were observed in IR group under light microsope and electron microscope, but there was only slight degeneration in IL lra group.Conclusion: The results indicated that IL 1ra were effective in protecting the isolated swine kidney from IR injury.

关 键 词：猪 离体肾 IL-1受体拮抗剂 缺血/再灌注损伤 

分 类 号：R699.2[医药卫生—泌尿科学] R692[医药卫生—外科学]